Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

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dc.authorid0000-0003-0352-1947
dc.authorid0000-0002-7199-583X
dc.contributor.authorAkgül Çağlar, Tuba
dc.contributor.authorGünal, Mehmet Yalçın
dc.contributor.authorTurhan, Mehmet Uğurcan
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorÇağavi, Esra
dc.date.accessioned2021-03-22T11:10:08Z
dc.date.available2021-03-22T11:10:08Z
dc.date.issued2021
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Sinirbilim Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Biyoloji ve Genetik Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.description.abstractRetrograde dyes are often used in basic research to investigate neuronal innervations of an organ. This article describes the experimental data on the application of retrograde dyes on the mouse heart in vivo and on the cardiac or neuronal cultures in vitro. By providing this information, cardiac or inneinnervations can be evaluated in vivo. Therefore, unknown cellular and molecular mechanisms and systemic interactions in the body can be investigated. In particular, we provided practical tips to lower mortality risks following the cardiac surgery and evaluated the staining capacity and fluorescent characteristics of the Di-8-ANEPPQ dye in the cardiac tissue and cell cultures. First, primary cultures of mouse nodose ganglia (NG) neurons and mouse neonatal cardiomyocytes were stained with Di-8-ANEPPQ. The Di-8-ANEPPQ signal from live cultures were visualized using spinning disk confocal microscopy to verify the lipophilic and fluorescent labeling capacity of Di-8-ANEPPQ. Next, the excitation and emission data of Di-8-ANEPPQ were collected between 415 nm and 690 nm using power spectrum module of confocal microscopy. This spectrum analysis could be useful for the researchers who plan to use Di-8-ANEPPQ in combination with other fluorescent dyes to eliminate any florescent overlap. In order to label the heart tissue with tracer dyes Di-8-ANEPPQ or DiI in vivo, the heart was exposed without damaging lungs or other tissues following anesthetization, then the retrograde dye was applied as a paste for DiI or injected to the apex of the heart for Di-8-ANEPPQ and the operation area was sutured. The surgical procedure required intubation to control the respiratory reflex without the need to perform a tracheotomy and yielded high viability. Following labeling the heart in vivo, the heart was dissected, and images of injection area were captured using confocal microscopy. All fluorescent images of Di-8-ANEPPQ labeled cells were analyzed by using the Fiji software. Overall, these data provide applicable data to other investigators to trace the sensory neurons innervating not only the heart but also other organs using Di-8-ANEPPQ. These data support the original research article titled “Evaluation of bilateral cardiac afferent distribution at the spinal and vagal ganglia by retrograde labeling” that was accepted for publication in Brain Research Journal [1].
dc.identifier.citationAkgül Çağlar, T., Günal, M. Y., Turhan, M. U., Öztürk, G. ve Çağavi, E. (2021). Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo. Data in Brief, 35. https://dx.doi.org/10.1016/j.dib.2021.106834
dc.identifier.doi10.1016/j.dib.2021.106834
dc.identifier.issn2352-3409
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://dx.doi.org/10.1016/j.dib.2021.106834
dc.identifier.urihttps://hdl.handle.net/20.500.12511/6642
dc.identifier.volume35
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Inc.
dc.relation.ispartofData in Briefen_US
dc.relation.publicationcategoryDiğer
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/115S381
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectIn Vivo Labeling
dc.subjectDi-8-ANEPPQ
dc.subjectCardiac Afferents
dc.subjectRetrograde Tracers
dc.subjectIn Vitro Labeling
dc.subjectSensory System
dc.subjectCardiac System
dc.titleExperimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo
dc.typeOther

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