Evaluation of the in vitro anti-inflammatory activity of Nerium oleander L. flower extracts and activity-guided isolation of the active constituents

Basit Öğe Kaydı
dc.authorid0000-0002-7921-8621
dc.contributor.authorAtay Balkan, İrem
dc.contributor.authorGören, Ahmet Ceyhan
dc.contributor.authorKırmızıbekmez, Hasan
dc.contributor.authorYeşilada, Erdem
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:50:53Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:50:53Z
dc.date.issued2018
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Farmakognozi Ana Bilim Dalı
dc.descriptionWOS: 000416971500003
dc.description.abstractThe in vitro anti-inflammatory activity profile of the Nerium oleander flower EtOH extract/its subextracts (n-hexane, CH2Cl2, EtOAc, remaining H2O) were evaluated on LPS induced Raw 264.7 macrophages. The effects of the crude EtOH extract and its subextracts on nitric oxide (NO) production and cell viability were determined. The most active subextract was determined to be the EtOAc subextract without exerting any toxicity towards Raw 264.7 macrophages. This subextract significantly inhibited NO production of Raw 264.7 macrophages after LPS induction (62.56 +/- 1.91% at 200 mu g/mL concentration). The levels of iNOS were reduced up to 67.50%. Moreover, this subextract slightly reduced the phosphorylation levels of MAP kinases (p-ERK, p-JNK, p-38). The highest inhibition was observed for ERK phosphorylation, which was inhibited by 20.53% at 200 mu g/mL concentration. Through activity-guided fractionation procedures, kaempferol, kaempferol 3-O-beta-glucopyranoside and chlorogenic acid were isolated as the main active components. The structures of the active compounds were determined by 2D-NMR techniques and HRMS analysis. All compounds significantly inhibited NO productions. Results of the present study supported the traditional use of N. oleander flowers to treat inflammatory complaints.
dc.identifier.citationAtay Balkan, İ., Gören, A. C., Kırmızıbekmez, H. ve Yeşilada, E. (2018). Evaluation of the in vitro anti-inflammatory activity of Nerium oleander L. flower extracts and activity-guided isolation of the active constituents. Records of Natural Products, 12(2), 128-141. https://dx.doi.org/10.25135/rnp.15.17.05.100
dc.identifier.doi10.25135/rnp.15.17.05.100
dc.identifier.endpage141
dc.identifier.issn1307-6167
dc.identifier.issue2
dc.identifier.scopusqualityQ2
dc.identifier.startpage128
dc.identifier.urihttps://dx.doi.org/10.25135/rnp.15.17.05.100
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2096
dc.identifier.volume12
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherACG Publications
dc.relation.ispartofRecords of Natural Productsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectNerium Oleander
dc.subjectNitric Oxide
dc.subjectMitogen Activated Protein Kinases
dc.subjectKaempferol
dc.subjectKaempferol 3-O-Beta-Glucopyranoside
dc.subjectChlorogenic Acid
dc.titleEvaluation of the in vitro anti-inflammatory activity of Nerium oleander L. flower extracts and activity-guided isolation of the active constituents
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Balkan, Irem Atay-2018.pdf
Boyut:
1.08 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
Eczacılık Fakültesi Makale Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu