An integrated top-stage incubator and lens free holographic imaging system for culture monitoring applications

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dc.authorid0000-0003-0176-8807
dc.contributor.authorÖzbilgin, Zeynep
dc.contributor.authorAkşit, Sevdenur
dc.contributor.authorToy, Muhammed Fatih
dc.date.accessioned2022-07-19T06:12:30Z
dc.date.available2022-07-19T06:12:30Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Elektrik ve Elektronik Mühendisliği Bölümü
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractLens free inline holographic microscopy was shown to be a potent approach for many applications relying on cellular imaging. Applications requiring a large field of view at moderate resolution are the ones that are most suitable for this platform. Besides, the simplicity of the overall imaging system, which requires only a light source and a camera, positions this approach as an easily accessible one. Acquired holograms from such a system are processed to recover phase images. As an additional advantage on top of the simplicity, phase imaging enables the imaging of otherwise transparent cell samples without any need for labeling or staining. Eventually, such a system can be used for long term imaging of live cell cultures with wide field of view. Up to now, two alternatives were explored for the imaging of live cell cultures for extended duration. In one approach, a portable imaging system was placed inside a standard incubator with cell cultures on top. A stage top incubator was used on a modified microscope in the other approach. In both approaches, the cost of the system grows due to commercial systems, and the overall footprint of the system with incubator is too large to be classified as portable. Here, an integrated portable system is presented that can maintain cell cultures at desired temperature in a 3D printed enclosure while imaging them in lens free inline holographic microscopy modality. Such a system is well suited for tissue culturing and monitoring at limited resources settings.
dc.description.sponsorshipCity of Strasbourg ; CNRS ; Cube ; IdEx University of Strasbourg ; The Society of Photo-Optical Instrumentation Engineers (SPIE) ; Universite de Strasbourgen_US
dc.description.sponsorshipTurkish Academy of Sciences Outstanding Young Scientist Award Program (TUBA-GEBIP)en_US
dc.identifier.citationÖzbilgin, Z., Akşit, S. ve Toy, M. F. (2022). An integrated top-stage incubator and lens free holographic imaging system for culture monitoring applications. Unconventional Optical Imaging III. Virtual, Online, 9-20 May 2022. http://doi.org/10.1117/12.2622639
dc.identifier.doi10.1117/12.2622639
dc.identifier.isbn9781510651487
dc.identifier.issn0277-786X
dc.identifier.issn1996-756X
dc.identifier.scopus2-s2.0-85132836038
dc.identifier.scopusqualityN/A
dc.identifier.urihttp://doi.org/10.1117/12.2622639
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9583
dc.identifier.volume12136
dc.identifier.wos000837998500030en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorÖzbilgin, Zeynep
dc.institutionauthorToy, Muhammed Fatih
dc.language.isoen
dc.publisherSPIE
dc.relation.ispartofUnconventional Optical Imaging IIIen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCellular Imaging Systems
dc.subjectDigital In-Line Holographic Microscopy
dc.subjectGerchberg-Saxton Algorithm
dc.subjectLens Free Imaging
dc.subjectNumerical Reconstruction
dc.subjectTime-Lapse Microscopy
dc.subjectTop Stage Incubator
dc.titleAn integrated top-stage incubator and lens free holographic imaging system for culture monitoring applications
dc.typeConference Object

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