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Öğe Redox and lipidomic analysis of human brain microvascular endothelial cells (hcmec/d3) adapted to physiological oxygen levels(Elsevier Science Inc, 2024) Yang, Fan; Simon, Daniel; Eroğlu, Emrah; Aarsland, Dag; Siow, Richard C. M.; Mann, Giovanni E.The majority of studies with endothelial and other cell types are conducted in standard culture incubators gassed with 5% CO2 and room air (18 kPa O2), well known to induce oxidative stress.Öğe Pioneering precision: unraveling cellular bioenergetics with ph-control(2024) Ghaffari Zaki, Asal; Miri, Seyed Mohammad; Çimen, Şeyma; Akgül Çağlar, Tuba; Yiğit, Esra Nur; Aydın, Mehmet Şerif; Öztürk, Gürkan; Eroğlu, Emrah[Abstract Not Available]Öğe Development of a non-animal testing method using genetically engineered biosensors for assessing skin sensitizers(2024) Seçilmiş, Melike; Katı, Ahmet; Eroğlu, Emrah[Abstract Not Available]Öğe Hyper7 biosensor associate with proteomic shifts related to oxidative stress in endothelial cells(2024) Barakat, Sarah; Çimen, Şeyma; Miri, Seyed Mohammad; Vatandaşlar, Emre; Kök, Kıvanç; Öztürk, Gürkan; Eroğlu, Emrah[Abstract Not Available]Öğe Revisiting the role of h2o2 in nrf2 activation using an oxygen-independent biosensor(2024) Miri, Seyed Mohammad; Eroğlu, Emrah[Abstract Not Available]Öğe The relationship between mitochondrial oxidative stress and huntington's disease progression(2024) Yiğit, Esra Nur; Aydın, Mehmet Şerif; Eroğlu, Emrah; Öztürk, Gürkan[Abstract Not Available]Öğe Revisiting the role of h2o2 in nrf2 activation using an oxygenindependent biosensor(2024) Miri, Seyed Mohammad; Eroğlu, Emrah[Abstract Not Available]Öğe Decoding the implications of subcellular h2o2 dynamics in a stroke-on-a-dish model using cerebral microvascular endothelial cells(2024) Kalkan Yazıcı, Merve; Kekeç, Enes Görkem; Üvak, Melike Tuğba; Eroğlu, Emrah[Abstract Not Available]Öğe Importance of physiological oxygen levels for endothelial redox signaling using Hyper 7(Elsevier Science Inc, 2023) Altun, Hamza Yusuf; Yang, Fan; Mann, Giovanni; Eroğlu, EmrahCellular redox status is tightly linked to ambient oxygen levels. Endothelial cells (EC) in vivo are exposed to O2 levels between 3- 13 kPa, yet the majority in vitro studies maintain EC under atmospheric O2 levels (~18 O2). Under 18 kPa O2, EC are exposed to hyperoxia, leading to persistent upregulation of NRF2 regulated antioxidant defences. Exposure of EC to physiological O2 levels for at least 5 days attenuates NRF2-regulated antioxidant gene expression in the absence of HIF-1a stabilisation.Öğe Reactivity of posterior cortical electroencephalographic alpha rythms during eyes opening in cognitively intact older adults and patients with dementia due to Alzheimer's and lewy diseases(Elsevier, 2023) Lopez, S.; Babiloni, C.; Noce, G.; Tucci, F.; Lizio, R.; Ferri, R.; Soricelli, A.; del Percio, C.Introduction: Patients with Lewy Bodies (DLB) and Parkinson’s disease dementia (PDD) suffer from vigilance dysregulation reflected by hypersomnolence, cognitive fluctuations, hallucinations, and sleep disorders. Therefore, we hypothesized that such dysregulation may be reflected by altered reactivity of posterior resting-state electroencephalographic (rsEEG) alpha rhythms during the vigilance transition from eyes-closed to -open condition. More specifically, we tested whether the above mentioned reactivity of posterior rsEEG alpha rhythms may differ in patients with DLB, PDD and Alzheimer’s disease (ADD) dementia, as a functional probe of the dominant cortical neural synchronization mechanisms regulating the vigilance in posterior visual systems. Methods: In two different studies, we extracted clinical, demographical, and rsEEG datasets in (1) older adults (Healthy, N=28) seniors as well as matched DLB (N=42) and ADD (N=48) patients, and (2) 25 Healthy seniors as well as matched PDD (N=73) and ADD (N=35) patients, from an international Eurasian database. The eLORETA freeware was used to estimate cortical rsEEG sources at delta, theta, alpha, beta, and gamma frequency bands during eye-closed and eye-open conditions.Öğe Resting state electroencephalographic alpha rhythms predict and are sensitive to Alzheimer's disease mild cognitive impairment progression at a 6-month follow-up(Elsevier, 2023) Babiloni, C.; Noce, G.; del Percio, Claudio; Tucci, F.; Lopez, S.; Ferri, R.; Soricelli, A.; Arnaldi, D.; Giubilei, F.; Stocchi, F.; Güntekin, Bahar; Yener, Görsev; Taylor, J. P.; Pantano, P.; Piervincenzi, Claudia; Nikolaos, Petsas; Frisoni, Giovanni B.; Jakhar, DeepakIntroduction: Patients with Alzheimer’s disease and mild cognitive impairment (ADMCI) suffer not only from severe cognitive deficits and disabilities in daily living but also from vigilance dysregulation reflected by substantial hypersomnolence due to vigilance fluctuations. Therefore, we hypothesized that such vigilance dysregulation in ADMCI patients might be reflected by altered electroencephalographic (rsEEG) rhythms recorded in a resting-state eyes-closed condition in relation to the disease progression at 6-month follow-ups.Öğe Event-related brain oscillatory responses topographically differentiated between visual and auditory emotional stimuli(Elsevier, 2023) Dalmızrak, Esra; Güntekin, BaharPrevious EEG studies using emotional stimuli have successfully demonstrated that EEG-brain oscillation responses are sensitive to the valance and arousal of the stimulus. While the valance is represented by increased beta and gamma responses, arousal is represented by increased delta and theta responses. Although the event-related oscillations during visual-emotional paradigms are relatively well-established, the differentiation between visual and auditory emotional stimulation is not fully understood yet.Öğe Chemogenetic tools and genetic biosensors in redox biology: Probing Hyper7 signals in specific cellular locales(Elsevier Science Inc., 2023) Altun, Hamza Yusuf; Erdoğan, Yusuf Ceyhun; Seçilmiş, Melike; Eroğlu, EmrahH2O2 is one of the most studied reactive oxygen species (ROS) in redox biology because of its critical role in oxidant-dependent signaling pathways. Conventional approaches apply exogenous H2O2 on cells and tissues, which barely recapitulates intracellular redox signaling events. An alternative approach is D-amino acid oxidase (DAAO)-based chemogenetic tools that permit intracellular H2O2 generation by D-amino acid catalysis. Exploiting the targetability of the DAAOs to cellular ultralocales allow precise manipulation of the redox tone with high spatial and temporal resolution. DAAOs have been utilized in several redox studies, yet due to the incomplete characterization and the lack of fine-tuned protocol on how to employ DAAOs, the potential of this multiparametric imaging technology could not be fully exploited. Using the ultra-sensitive genetically encoded H2O2 biosensor (HyPer), we tested the performance of a modified version of DAAO (mDAAO) under hypoxic and hyperoxic conditions. Our multiparametric imaging approach unveiled that cell type, cellular locales, pericellular oxygen concentrations, D-amino acid type, and its concentrations are critical and modulatable factors that permit fine-tuned H2O2 generation with high precision. These findings and fine-tuned protocols provide practical approaches for redox scientists to spatiotemporally control oxidative stress-dependent signaling pathways in different cultured cell model systems.Öğe An integrated top-stage incubator and lens free holographic imaging system for culture monitoring applications(SPIE, 2022) Özbilgin, Zeynep; Akşit, Sevdenur; Toy, Muhammed FatihLens free inline holographic microscopy was shown to be a potent approach for many applications relying on cellular imaging. Applications requiring a large field of view at moderate resolution are the ones that are most suitable for this platform. Besides, the simplicity of the overall imaging system, which requires only a light source and a camera, positions this approach as an easily accessible one. Acquired holograms from such a system are processed to recover phase images. As an additional advantage on top of the simplicity, phase imaging enables the imaging of otherwise transparent cell samples without any need for labeling or staining. Eventually, such a system can be used for long term imaging of live cell cultures with wide field of view. Up to now, two alternatives were explored for the imaging of live cell cultures for extended duration. In one approach, a portable imaging system was placed inside a standard incubator with cell cultures on top. A stage top incubator was used on a modified microscope in the other approach. In both approaches, the cost of the system grows due to commercial systems, and the overall footprint of the system with incubator is too large to be classified as portable. Here, an integrated portable system is presented that can maintain cell cultures at desired temperature in a 3D printed enclosure while imaging them in lens free inline holographic microscopy modality. Such a system is well suited for tissue culturing and monitoring at limited resources settings.Öğe Maskless lithography with holographic feedback for the fabrication of optical elements(SPIE, 2022) Gürcan, Tolga; Toy, Muhammed FatihPhotolithography has become a powerful tool in the fabrication of micro-optical elements following the advancements in grayscale approaches. However, hitting tight design tolerance goals require precise control of all parameters such as temperature, resist nonlinearity or preventing vignetting. In this work we took an alternative route to these problems by combining maskless lithography with digital holography. Addition of digital holography enables the use of feedback by measuring the quantitative phase of specimen near real time and in situ nondestructively. After each near UV exposure, phase retardation map of exposed photopolymer is measured with digital holography part of the system. Any deviation from target phase is corrected by changing the pattern displayed on the mask. We showed that the proposed method reduces the standard deviation of resulting phase compared to traditional one-shot grayscale lithography. It also does not require any precalibration of photoresist and relaxes the constraints for uniform UV illumination in sample plane.Öğe Dual function of HSP70 in cytoprotection of tumor cells and generation of immunosuppressive tumor microenvironment(American Association for Cancer Research, 2021) Korkaya, Hasan; Köksalar Alkan, Fulya; Ece Arslan, Z.; Altıntaş, Esra; Piranlıoğlu, Raziye; Lee, Eunmi; Wicha, Max S.[Abstract Not Available]Öğe Prognostic significance of SERPINB1 expression in gliomas(Wiley, 2021) Uludağ, D.; Karaoğlan, B. S.; Genç, B.; Tahta, A.; Mohr, T.; Çakır, A.; Mayliba, H. B.; Karakaş, N.Glioblastoma (GB) is the most prevalent and aggressive brain tumor with high morbidity. Determination of tumor initiative and prognostic factors are ultimately needed for therapy options. The effects of Serine Protease Inhibitor (SERPIN) B1 on tumor progression have been reprted in several cancer types including breast and lung cancers. However, the relation between SERPINB1 expression levels and glioma progression is still to be elucidated.
