Evaluation of in vitro anti-inflammatory effects of the remaining water subextract of Cistus laurifolius leaves

Basit Öğe Kaydı
dc.contributor.authorAtay, İrem
dc.contributor.authorİlter, Ayça Zeynep
dc.contributor.authorBagatur, Yeşim
dc.contributor.authorTelci, Dilek
dc.contributor.authorKırmızbekmez, Hasan
dc.contributor.authorYeşilada, Erdem
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:02:34Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:02:34Z
dc.date.issued2015
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Farmakognozi Ana Bilim Dalı
dc.description63rd International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research (GA) -- August 23-27, 2015 -- Budapest, Hungary
dc.descriptionWOS: 000367558100415
dc.description.abstractCistus laurifolius L. (Cistaceae) is known as 'laden' in Anatolia and the leaves of the plant are used against rheumatismal diseases traditionally [1]. The ethanol extract of the leaves were submitted to solvent-solvent extractions and n-hexane, chloroform, ethyl acetate, n-butanol, remaining water subextracts were obtained. The extract and subextracts were investigated for their inhibitory effects on Nuclear Factor kappa B (NF-?B) transcrption factor on lipopolysaccharide induced Raw 264.7 macrophages. Only remaining water subextract exerted 20% inhibition. The subextract was fractionated by chromatographic methods but the fractions were found to be not effective. Thus, remaining water subextract was directed for further in vitro anti-inflammatory investigations. The effects on nitric oxide (NO), prostaglandine E2 (PGE2), tumor necrosis factor (TNF?) and interleukins (IL-1?, IL-1?, IL-2, IL-6) were studied by Griess and ELISA. The effects on iNOS and COX-2 protein levels and the phosphorylation levels of mitogen activated protein kinases (ERK1/2, JNK, p38) and I kappa B alpha (I?B?) were examined by Western Blot method. The subextract was applied to Raw 264.7 macrophage cells at 25, 50 and 100 µg/mL concentrations and it provided up to 57% decrease of NO production and up to 55% decrease of PGE2 production of cells. The iNOS and COX-2 protein levels were decreased accordingly. The subextract exerted inhibitory effect on TNF? (37%). This subextract inhibited the phosphorylation of I?B? at 50 and 100 µg/mL concentrations while JNK phosphorylation was concentration-dependently inhibited.
dc.identifier.citationAtay, İ., İlter, A. Z., Bagatur, Y., Telci, D., Kırmızbekmez, H. ve Yeşilada, E. (2015). Evaluation of in vitro anti-inflammatory effects of the remaining water subextract of Cistus laurifolius leaves. 63rd International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research içinde (1511-1511. ss.). Budapest, Hungary, August 23-27, 2015.
dc.identifier.endpage1511
dc.identifier.issn0032-0943
dc.identifier.issn1439-0221
dc.identifier.issue16
dc.identifier.startpage1511
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3681
dc.identifier.volume81
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherGeorg Thieme Verlag
dc.relation.ispartof63rd International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Researchen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEvaluation
dc.subjectSubextract
dc.subjectCistus
dc.titleEvaluation of in vitro anti-inflammatory effects of the remaining water subextract of Cistus laurifolius leaves
dc.typeConference Object

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Eczacılık Fakültesi Bildiri Koleksiyonu