Whole-mount RNA in situ hybridization of zebrafish embryos

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dc.authorid0000-0002-6274-801X
dc.contributor.authorÜnal, İsmail
dc.contributor.authorCansız, Derya
dc.contributor.authorBeler, Merih
dc.contributor.authorAlturfan, Ahmet Ata
dc.contributor.authorEmekli Alturfan, Ebru
dc.date.accessioned2024-02-20T13:22:03Z
dc.date.available2024-02-20T13:22:03Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractA commonly employed technique in molecular biology to evaluate the temporal and spatial expression of a certain gene is in situ hybridization. This method is an effective strategy to construct synexpression groups, co-expressed genes acting in shared biological processes, and to find new members of genes engaged in the same signaling pathways to discover similar spatial and temporal expression patterns in zebrafish embryos. The major disadvantage of this method is that RNA probes can penetrate within 2 days of post-fertilization embryos, and therefore, in later developmental stages, the probe can only reach the surface tissues. Further application of the method in histological sections will be required for a complete and accurate gene expression investigation. However, this method is highly effective at late embryogenesis and early larval stages for observing gene expression in endodermal derivatives and sensory organs. RNA probes for in situ hybridization can be prepared through in vitro transcription from plasmids carrying specific promoter elements and mRNA-specific cDNA, or an alternative polymerase chain reaction (PCR) method can be used through PCR amplification. This chapter describes the procedures for detecting gene expression in zebrafish embryos using whole-mount RNA in situ hybridization.
dc.identifier.citationÜnal, İ., Cansız, D., Beler, M., Alturfan, A. A. ve Emekli Alturfan, E. (2024). Whole-mount RNA in situ hybridization of zebrafish embryos. Methods in Molecular Biology, 2753, 543-551. https://dx.doi.org/10.1007/978-1-0716-3625-1_35
dc.identifier.doi10.1007/978-1-0716-3625-1_35
dc.identifier.endpage551
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.pmid38285366
dc.identifier.scopus2-s2.0-85183776133
dc.identifier.scopusqualityQ3
dc.identifier.startpage543
dc.identifier.urihttps://dx.doi.org/10.1007/978-1-0716-3625-1_35
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12293
dc.identifier.volume2753
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCansız, Derya
dc.language.isoen
dc.publisherHumana Press Inc.
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararası
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIn Situ Hybridization
dc.subjectPolymerase Chain Reaction
dc.subjectRNA Probe
dc.subjectZebrafish Embryo
dc.titleWhole-mount RNA in situ hybridization of zebrafish embryos
dc.typeBook Chapter

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