Online-HPLC strategies, antioxidant and enzyme inhibitory activities of different extracts of mondia whitei leaves

Basit Öğe Kaydı
dc.authorid0000-0002-4449-0537
dc.contributor.authorAhmed, Shakeel
dc.contributor.authorSinan, Kouadio Ibrahime
dc.contributor.authorNilofar, Claudio
dc.contributor.authorFerrante, Claudio
dc.contributor.authorEyupoğlu, Ozan Emre
dc.contributor.authorEtienne, Ouattara Katinan
dc.contributor.authorZengin, Gökhan
dc.date.accessioned2023-12-25T07:44:55Z
dc.date.available2023-12-25T07:44:55Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümü, Biyokimya Ana Bilim Dalı
dc.description.abstractBackground: Mondia whitei, an indigenous African plant, is widely recognised for its medicinal properties. Throughout ancient times, African communities have employed this remedy to address a wide range of health conditions. However, there is a lack of online High-Performance Liquid Chromatography (HPLC) studies available for this plant. The present investigation aimed to address and alleviate this deficiency by providing a comprehensive analysis.Methods: Methanol, combination of water and methanol, ethyl acetate, and water extracts of Mondia whitei (M. whitei) leaves were utilized in this study. The phenolic and flavonoids composition of the extracts determined by Folin-Ciocalteu and Aluminum chloride (AlCl3) assays, respectively. The antioxidant potential of all the extracts was assessed through a range of in vitro assays, including FRAP (Ferric reducing antioxidant power), DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS [2,2 '-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)], CUPRAC (cupric reducing antioxidant capacity), PBD (phosphomolybdenum), and MCA (metal chelating ability). Subsequently, the online HPLC-linked system had established to compare these extracts using four distinct antioxidant analysis methods: FRAP, DPPH, ABTS, and CUPRAC. The enzyme inhibitory properties were also investigated against cholinesterases, tyrosinase, alpha-amylase and alpha-glucosidase.Results: The methanol-water and methanol extracts had the highest phenolic contents and demonstrated superior efficacy as scavenging/reducing agents and enzyme inhibitors, particularly against tyrosinase and cholinesterase (p < 0.05). Through the utilisation of various online HPLC antioxidant techniques, the identification of four molecules exhibiting antioxidant activity was achieved. These compounds were identified as Coumarin, Vanillin, p-OH benzoic acid, and Chlorogenic acid.Conclusions: Mondia whitei leaves can be regarded as a valuable source of natural bioactive compounds, with the potential to serve as a material basis for pharmacological effects in nutraceutical and pharmaceutical applications.
dc.identifier.citationAhmed, S., Sinan, K. I., Nilofar, C., Ferrante, C., Eyupoğlu, O. E., Etienne, O. K. ... Zengin, G. (2023). Online-HPLC strategies, antioxidant and enzyme inhibitory activities of different extracts of mondia whitei leaves. Journal of Biological Regulators and Homeostatic Agents, 37(11), 6029-6039. https://dx.doi.org/10.23812/j.biol.regul.homeost.agents.20233711.577
dc.identifier.doi10.23812/j.biol.regul.homeost.agents.20233711.577
dc.identifier.endpage6039
dc.identifier.issn0393-974X
dc.identifier.issn1724-6083
dc.identifier.issue11
dc.identifier.startpage6029
dc.identifier.urihttps://dx.doi.org/10.23812/j.biol.regul.homeost.agents.20233711.577
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12066
dc.identifier.volume37
dc.identifier.wos001114250800001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.institutionauthorEyupoğlu, Ozan Emre
dc.language.isoen
dc.publisherBiolife Sas
dc.relation.ispartofJournal of Biological Regulators and Homeostatic Agentsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectMondia Whitei
dc.subjectOnline HPLC Assays
dc.subjectAntioxidants
dc.subjectEnzyme Inhibitors
dc.titleOnline-HPLC strategies, antioxidant and enzyme inhibitory activities of different extracts of mondia whitei leaves
dc.typeArticle

Dosyalar

Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Eczacılık Fakültesi Makale Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu