Assessment of the MRI and behavioral test results in a focal cerebral ischemia-reperfusion model in the rat after separate and combined use of mouse-derived neural progenitor cells, human-derived neural progenitor cells and atorvastatin

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dc.authorid0000-0003-0467-7521
dc.contributor.authorTahta, Alican
dc.contributor.authorİzgi, Nail
dc.contributor.authorBağcı Önder, Tuğba
dc.contributor.authorErdağ, Ece
dc.contributor.authorAras, Yavuz
dc.contributor.authorGenç, Çetin
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:04:12Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:04:12Z
dc.date.issued2018
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı
dc.descriptionWOS: 000437251200010
dc.descriptionPubMed ID: 30192361
dc.description.abstractAIM: To assess the efficacy of Neural progenitor cell (NPC) transplantation in ischemic stroke, and to investigate whether atorvastatin enhances therapeutic potency of NPC after stroke. MATERIAL and METHODS: The focal cerebral ischemia-reperfusion model was performed by transient occlusion of middle cerebral artery. Rats were assigned randomly to receive intracerebral transplantation of mouse NPC alone (mNPC), human NPC alone (hNPC), mouse NPC plus oral atorvastatin (mNPC+A), human NPC plus oral atorvastatin (hNPC+A), oral atorvastatin alone, or intracerebral Dulbecco's Modified Eagle's medium injection (control group). Adhesive removal, rotarod, cylinder tests, and magnetic resonance imaging (MRI) were used for assessment of rats during 4 weeks. After sacrification on 28th day, rats were investigated by immunofluorescent staining. RESULTS: The hNPC and mNPC groups showed significantly improved functional outcome and reduced infarct area ratio compared with the control group. The hNPC group had significantly better performance and lower infarct area ratio than the mNPC group. Addition of atorvastatin to stem cell therapy significantly improved functional outcome, although it did not affect the infarct area ratio on MRI. Anti-inflammatory response in the infarct area was higher in the mNPC group. NPC transplantation significantly reduced the amount of microglia and a significant increase in the amount of astrocytes. CD8a+ T lymphocyte and granzyme B activities were not detected in any of the subjects. CONCLUSION: Both hNPC and mNPC treatments significantly improved functional outcome, and reduced infarct area ratio after stroke. Atorvastatin enhanced the therapeutic potency of NPCs, including neurological improvement.
dc.description.sponsorshipIstanbul University Scientific Research Projects [50529]en_US
dc.description.sponsorshipWe thank Ahmet Cingoz for assistance with NPC culture. This work was supported by Istanbul University Scientific Research Projects under Grant 50529.en_US
dc.identifier.citationTahta, A., İzgi, N., Bağcı Önder, T., Erdağ, E., Aras, Y. ve Genç, Ç. (2018). Assessment of the MRI and behavioral test results in a focal cerebral ischemia-reperfusion model in the rat after separate and combined use of mouse-derived neural progenitor cells, human-derived neural progenitor cells and atorvastatin. Turkish Neurosurgery, 28(4), 571-581. https://dx.doi.org/10.5137/1019-5149.JTN.21789-17.1
dc.identifier.doi10.5137/1019-5149.JTN.21789-17.1
dc.identifier.endpage581
dc.identifier.issn1019-5149
dc.identifier.issue4
dc.identifier.scopusqualityQ3
dc.identifier.startpage571
dc.identifier.urihttps://dx.doi.org/10.5137/1019-5149.JTN.21789-17.1
dc.identifier.urihttps://hdl.handle.net/20.500.12511/4013
dc.identifier.volume28
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Neurosurgical Society
dc.relation.ispartofTurkish Neurosurgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAtorvastatin
dc.subjectFocal Cerebral Ischemia
dc.subjectHuman Derived Neural Progenitor Cell
dc.subjectMouse Derived Neural Progenitor Cell
dc.subjectNeural Progenitor Cell
dc.subjectRats
dc.titleAssessment of the MRI and behavioral test results in a focal cerebral ischemia-reperfusion model in the rat after separate and combined use of mouse-derived neural progenitor cells, human-derived neural progenitor cells and atorvastatin
dc.typeArticle

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