Melatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats

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dc.authorid0000-0002-2018-9619
dc.contributor.authorAydemir, Sezgin
dc.contributor.authorAkgün, Sevcan Gül
dc.contributor.authorBeceren, Ayfer
dc.contributor.authorYüksel, Meral
dc.contributor.authorKumaş, Meltem
dc.contributor.authorErdoğan, Nusret
dc.contributor.authorSardaş, Semra
dc.contributor.authorOmurtag, Gülden Zehra
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:56:34Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:56:34Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Farmasötik Toksikoloji Ana Bilim Dalı
dc.descriptionWOS: 000400553200039
dc.description.abstractFormaldehyde (FA) is an organic chemical which is widely used all over the world and has hazardous effects for the environment. FA can react with many biomolecules in the biological systems and lead to toxic effects on humans. Melatonin (MEL), a neurohormone produced by pineal gland, has been shown to be an effective antioxidant with free radical scavenging properties. The present study aimed to evaluate the ameliorative effects of MEL on FA-induced toxicity by monitoring oxidant/antioxidant and histopathological changes in the lung, liver and kidney tissues of rats as well as DNA damage in the blood samples. FA was administered through inhalation at a rate of 6 ppm for 6 weeks and intraperitoneal injection at a rate of 10 mg/kg/day for 14 days. MEL was administered in related groups at a rate of 10 mg/kg/day. Upon the completion of the experimental protocol, tissues were dissected for processing biochemical assays and routine histological staining. Blood samples were collected to investigate DNA damage with the comet assay and ELISA kit for 8-hydroxydeoxyguanosine (8-OHdG). FA exposures increased the levels of DNA damage, malondialdehyde and myeloperoxidase activity and reduced glutathione levels. FA also significantly raised the level of tissue reactive oxygen species. FA-induced morphological changes in the tissues were also observed with the light microscope. These alterations were reversed by MEL treatment. In conclusion, the present study suggests that oxidative mechanisms play an important role in FA toxicity. MEL ameliorates oxidative tissue and DNA damage resulting from FA-induced toxicity by balancing oxidant-antioxidant status, inhibiting neutrophil infiltration and reducing 8-OHdG level, and might be beneficial in reducing FA-induced oxidative tissue and DNA damage.
dc.description.sponsorshipCommission of Scientific Investigations Projects (BAPKO) of Marmara University [SAG-C-DRP-110915-0414]en_US
dc.description.sponsorshipThis study was financially supported by Grant Project No: SAG-C-DRP-110915-0414 from the Commission of Scientific Investigations Projects (BAPKO) of Marmara University.en_US
dc.identifier.citationAydemir, S., Akgün, S. G., Beceren, A., Yüksel, M., Kumaş, M., Erdoğan, N. … Omurtag, G. Z. (2017). Melatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats. International Journal of Clinical and Experimental Medicine, 10(4), 6250-6261.
dc.identifier.endpage6261
dc.identifier.issn1940-5901
dc.identifier.issue4
dc.identifier.scopusqualityQ3
dc.identifier.startpage6250
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2751
dc.identifier.volume10
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherE-Century Publishing Corp
dc.relation.ispartofInternational Journal of Clinical and Experimental Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFormaldehyde
dc.subjectMelatonin
dc.subjectOxidative Stress
dc.subjectChemiluminescence
dc.subjectComet Assay
dc.subjectDNA Damage
dc.subjectHistopathology
dc.titleMelatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats
dc.typeArticle

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