Chemogenetic approaches to dissect the role of H2O2 in redox-dependent pathways using genetically encoded biosensors

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dc.authorid0000-0003-2033-860X
dc.authorid0000-0002-9373-0808
dc.contributor.authorGhaffari Zaki, Asal
dc.contributor.authorErdoğan, Yusuf Ceyhun
dc.contributor.authorAkgül Çağlar, Tuba
dc.contributor.authorEroğlu, Emrah
dc.date.accessioned2022-03-18T08:01:41Z
dc.date.available2022-03-18T08:01:41Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractChemogenetic tools are recombinant enzymes that can be targeted to specific organelles and tissues. The provision or removal of the enzyme substrate permits control of its biochemical activities. Yeast-derived enzyme D-amino acid oxidase (DAAO) represents the first of its kind for a substrate-based chemogenetic approach to modulate H2O2 concentrations within cells. Combining these powerful enzymes with multiparametric imaging methods exploiting genetically encoded biosensors has opened new lines of investigations in life sciences. In recent years, the chemogenetic DAAO approach has proven beneficial to establish a new role for (patho)physiological oxidative stress on redoxdependent signaling and metabolic pathways in cultured cells and animal model systems. This mini-review covers established or emerging methods and assesses newer approaches exploiting chemogenetic tools combined with genetically encoded biosensors.
dc.identifier.citationGhaffari Zaki, A., Erdoğan, Y. C., Akgül Çağlar, T. ve Eroğlu, E. (2022). Chemogenetic approaches to dissect the role of H2O2 in redox-dependent pathways using genetically encoded biosensors. Biochemical Society Transactions, 50(1), 335-345. https://doi.org/10.1042/BST20210506
dc.identifier.doi10.1042/BST20210506
dc.identifier.endpage345
dc.identifier.issn0300-5127
dc.identifier.issn1470-8752
dc.identifier.issue1
dc.identifier.pmid35015078
dc.identifier.scopus2-s2.0-85125554344
dc.identifier.scopusqualityQ1
dc.identifier.startpage335
dc.identifier.urihttps://doi.org/10.1042/BST20210506
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9139
dc.identifier.volume50
dc.identifier.wos000743356700001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAkgül Çağlar, Tuba
dc.institutionauthorEroğlu, Emrah
dc.language.isoen
dc.publisherPortland Press Ltd
dc.relation.ispartofBiochemical Society Transactionsen_US
dc.relation.publicationcategoryDiğer
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/118C242
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectChemogenetics
dc.subjectGenetically Encoded Biosensors
dc.subjectReactive Nitrogen Species
dc.subjectReactive Oxygen Species
dc.subjectSignalling
dc.titleChemogenetic approaches to dissect the role of H2O2 in redox-dependent pathways using genetically encoded biosensors
dc.typeReview Article

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