Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?

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dc.authorid0000-0003-2003-6337
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorİşyar, Mehmet
dc.contributor.authorÖznam, Kadir
dc.contributor.authorMahiroğulları, Mahir
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:51:58Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:51:58Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Farmakoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Ortopedi ve Travmatoloji Ana Bilim Dalı
dc.descriptionWOS: 000415126000002
dc.descriptionPubMed ID: 29125811
dc.description.abstractObjectives: This study aims to investigate the possible effects of leukocyte concentration in the content of platelet-rich plasma (PRP) and the administration of PRP using a drug delivery system on chondrocyte proliferation in vitro conditions. Patients and methods: Blood from nine male patients (mean age 65 years; range 49 to 81 years) with advanced stage osteoarthritis who had not responded to medical or conservative treatments and underwent total knee arthroplasty was used to prepare two formulations: PRP with low concentration leukocytes (2000-4000 leukocytes/mu L) was designated as pure PRP (P-PRP), whereas PRP with high concentration leukocytes (9000-11000 leukocytes/mu L) as leukocyte-rich PRP (L-PRP). Samples were divided into five groups as control group (group 1), chondrocyte cultures with P-PRP applied directly (group 2), chondrocyte cultures with L-PRP applied directly (group 3), chondrocytes co-cultured with P-PRP applied hydrogel (group 4), and chondrocytes co-cultured with L-PRP applied hydrogel (group 5). In all groups; cell morphology, viability and proliferation were compared with the expression of stage-specific embryonic antigen-1 (SSEA-1), a precondrocyte marker. Results: Maximum cell proliferation and SSEA-1 expression occurred in group 4, with a statistically significant correlation between SSEA-1 expression and cell proliferation. Conclusion: Our study showed the importance of leukocyte concentration of PRP and efficiency of delivery systems such as hydrogel and that L-PRP administered with a delivery system is more efficient than conventional applications of PRP in the treatment of cartilage damage.
dc.description.sponsorshipNamik Kemal University Scientific Research Administration Division [NKUBAP.00.10.AR.15.08]en_US
dc.description.sponsorshipThis study was supported by grants (Project no: NKUBAP.00.10.AR.15.08) from Namik Kemal University Scientific Research Administration Division.en_US
dc.identifier.citationYaşar Şirin, D., Yılmaz, İ., İşyar, M., Öznam, K. ve Mahiroğulları, M. (2017). Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation? Joint Diseases and Related Surgery, 28(3), 142-151. https://dx.doi.org/10.5606/ehc.2017.55186
dc.identifier.doi10.5606/ehc.2017.55186
dc.identifier.endpage151
dc.identifier.issn1305-8282
dc.identifier.issn1309-0313
dc.identifier.issue3
dc.identifier.scopusqualityQ2
dc.identifier.startpage142
dc.identifier.urihttps://dx.doi.org/10.5606/ehc.2017.55186
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2318
dc.identifier.volume28
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Joint Diseases Foundation
dc.relation.ispartofJoint Diseases and Related Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCo-Culture Techniques
dc.subjectHydrogel
dc.subjectPlatelet-Rich Plasma
dc.subjectStage-Specific Embryonic Antigens
dc.titleDoes leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?
dc.title.alternativeBiyopolimerler ile uygulanan lökositten fakir veya lökositten zengin trombositten zengin plazmanın kondrosit proliferasyonunda konvansiyonel trombositten zengin plazma uygulamalarına üstünlüğü var mıdır?
dc.typeArticle

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