Screening analysis of doping agents in horse urine and plasma with dilute and shoot using liquid chromatography high resolution mass spectrometry

Basit Öğe Kaydı
dc.contributor.authorGöktaş, Eylem Funda
dc.contributor.authorKabil, Erol
dc.contributor.authorSöylemez Yeşilçimen, Esma
dc.contributor.authorDirikolu, Levent
dc.date.accessioned2026-04-16T07:38:44Z
dc.date.available2026-04-16T07:38:44Z
dc.date.issued2025
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Farmasötik Toksikoloji Ana Bilim Dalı
dc.description.abstractVarious technical methodologies are required to accurately detect substances of different chemical and pharmacological properties in biological samples, which are increasing in number and variety daily. Therefore, laboratories where many samples and different factors are analyzed simultaneously need methods with easy sample preparation, short analysis times and low analysis costs. In this study, the objective was to scan substances susceptible to chemical degradation, amenable to analysis without hydrolysis, and exhibiting short-term stability by employing a straightforward, expeditious, and costefficient method. For this purpose, a high-throughput dilute and shoot screening protocol was developed and validated utilizing high-performance liquid chromatography coupled with high-resolution mass spectrometry to analyze various pharmacological compounds in horse urine and plasma. Over 200 prohibited substances across multiple categories were scanned within a 13 minute run. Chromatographic separation was performed on a C18 column using an elution gradient of mobile phase A, 5 mM ammonium bicarbonate at pH 9, and mobile phase B, methanol, at a flow rate of 0.3 mL min-1. The method was validated according to the specifications of 2002/657/EC multi-screening requirements. The detection capability ranged from ≤1 to 200 ng mL-1 for prohibited substances. The implementation of the screening method in doping analysis, and the analysis of real positive case samples served to underscore the practical applicability of the developed method. To the best of our knowledge, this is a rare method that can be applied to both urine and plasma samples and provides a rapid, practical, broad-spectrum, and high-throughput analysis of prohibited substances in horse plasma and urine cost-effectively.
dc.identifier.citationGöktaş, E. F., Kabil, E., Söylemez Yeşilçimen, E. ve Dirikolu, L. (2025). Screening analysis of doping agents in horse urine and plasma with dilute and shoot using liquid chromatography high resolution mass spectrometry. Analyst, 150(4), 773-792. http://dx.doi.org/10.1039/d4an01501k
dc.identifier.doi10.1039/d4an01501k
dc.identifier.endpage792
dc.identifier.issn0003-2654
dc.identifier.issn1364-5528
dc.identifier.issue4
dc.identifier.pmid39846867
dc.identifier.scopus2-s2.0-85216323275
dc.identifier.scopusqualityQ1
dc.identifier.startpage773
dc.identifier.urihttp://dx.doi.org/10.1039/d4an01501k
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13431
dc.identifier.volume150
dc.identifier.wosWOS:001402511400001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSöylemez Yeşilçimen, Esma
dc.institutionauthorid0000-0002-6309-7546
dc.language.isoen
dc.relation.ispartofAnalyst
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnimals
dc.subjectChromatography
dc.subjectHigh Pressure Liquid
dc.subjectDoping in Sports
dc.subjectHorses
dc.subjectMass Spectrometry
dc.subjectSubstance Abuse Detection
dc.titleScreening analysis of doping agents in horse urine and plasma with dilute and shoot using liquid chromatography high resolution mass spectrometry
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Söylemez Yeşilçimen-Esma-2025.pdf
Boyut:
2.26 MB
Biçim:
Adobe Portable Document Format
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.17 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Eczacılık Fakültesi Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu