Dispersive solid phase extraction and quadruple isotope dilution-mass spectrometry combination for the accurate and sensitive quantification of capsaicin in pepper, domestic wastewater and human saliva samples by gc-ms system

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dc.contributor.authorBodur, Süleyman
dc.contributor.authorErarpat Bodur, Sezin
dc.contributor.authorGürsoy, Selim
dc.contributor.authorFırat Ayyıldız, Merve
dc.contributor.authorKartoğlu, Bedrihan
dc.contributor.authorAkbıyık, Hilal
dc.contributor.authorBakırdere, Sezgin
dc.date.accessioned2025-12-01T13:09:14Z
dc.date.available2025-12-01T13:09:14Z
dc.date.issued2025
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalı
dc.description.abstractIn the presented study, reduced graphene oxide/Fe3O4 (rGO/Fe3O4) nanocomposites based dispersive solid phase extraction (DSPE) – gas chromatography–mass spectrometry (GC–MS) method was developed for the determination of capsaicin in domestic wastewater (DW), pepper (PP) and human saliva (HS) samples. All important parameters of the DSPE method affected the preconcentration factor were carefully optimized to achieve high signal to noise ratio for the analyte. After the optimization studies, the system analytical performance of DSPE-GC–MS system was evaluated using the aqueous standard solution of capsaicin. Limit of detection (LOD), limit of quantitation (LOQ) and dynamic range were figured out to be 0.54 µg/kg, 1.80 µg/kg and 2.66 – 487.35 µg/kg, respectively. Under the optimum experimental conditions, recovery studies were conducted with the spiked DW, PP and HS samples, and percent recovery results were recorded between 52.6 % and 183.6 % via matrix matching calibration strategy. After the implementation of ID4 strategy, percent recovery results for the spiked DW, PP and HS samples were calculated as 98.2 %–99.3 %, 99.7 %–100.7 % and 99.4 %–99.8 %, respectively. In addition, capsaicin content in Sivri (S)-PP, Kıl (K)-PP and Samandağ (SA)-PP samples were found to be 309.5 ± 11.8 mg/kg, 873.7 ± 26.7 mg/kg and 165.3 ± 5.1 mg/kg via DSPE-GC-ID4-MS method, respectively. As a result, the combination of quadruple isotope dilution (ID4) strategy and the DSPE-GC–MS method were successfully performed to boost the accuracy and precision of developed DSPE-GC–MS method.
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)
dc.identifier.citationBodur, S., Erarpat Bodur, S., Gürsoy, S., Fırat Ayyıldız, M., Kartoğlu, B., Akbıyık, H. ... Bakırdere, S. (2025). Dispersive solid phase extraction and quadruple isotope dilution-mass spectrometry combination for the accurate and sensitive quantification of capsaicin in pepper, domestic wastewater and human saliva samples by gc-ms system. Microchemical Journal, 208. http://dx.doi.org/10.1016/j.microc.2024.112246
dc.identifier.doi10.1016/j.microc.2024.112246
dc.identifier.issn0026-265X
dc.identifier.issn1095-9149
dc.identifier.scopus2-s2.0-85210536558
dc.identifier.scopusqualityQ1
dc.identifier.urihttp://dx.doi.org/10.1016/j.microc.2024.112246
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13260
dc.identifier.volume208
dc.identifier.wosWOS:001370602800001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorGürsoy, Selim
dc.language.isoen
dc.relation.ispartofMicrochemical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/122Z041
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectCapsaicin
dc.subjectDispersive Solid Phase Extraction
dc.subjectDomestic Wastewater
dc.subjectPepper
dc.subjectQuadruple Isotope Dilution
dc.titleDispersive solid phase extraction and quadruple isotope dilution-mass spectrometry combination for the accurate and sensitive quantification of capsaicin in pepper, domestic wastewater and human saliva samples by gc-ms system
dc.typeArticle

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