In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts

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dc.authorid0000-0001-7277-920X
dc.authorid0000-0001-9265-8042
dc.authorid0000-0003-1896-2729
dc.authorid0000-0002-3412-0807
dc.contributor.authorÇaşkurlu, Ayşegül
dc.contributor.authorKaravuş, Şule Nur
dc.contributor.authorBiltekin, Sevde Nur
dc.contributor.authorHelvacı, Esra Zeynep
dc.contributor.authorKaradağ, Ayşe Esra
dc.date.accessioned2023-10-02T09:05:07Z
dc.date.available2023-10-02T09:05:07Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Farmakognozi Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümü, Farmasötik Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractThe aim of this study was to evaluate phytochemical composition of Ajuga reptans L. (Lamiaceae) aerial parts (separately flower and leaf) methanol, aqueous-methanolic extracts, and their cytotoxic activities. The phytochemical analysis was performed high-performance liquid chromatography (HPLC). Caffeic acid, p-coumaric, gallic, chlorogenic, ferulic acids, kaempferol, rutin, quercetin, quercetin-3-O-galactoside, and quercitrin were used as reference substances by HPLC in all samples. The major compounds in the extract were found as ferulic acid, caffeic acid, rutin, and quercetin-3-O-galactoside. Cytotoxicity was investigated using methyl thiazole tetrazolium (MTT) assay. Cytotoxic evaluation of the extracts against cancer (MCF7, PC3, and A549) and healthy human embryonic kidney cell line (HEK293) cell lines by MTT. Compared to other cells, the methanol extract of A. reptans demonstrated high selectivity against PC3 cells (IC50: 95 ± 0.99 µg/mL) and selectivity index was four times higher than reference drug colchicine. (IC50: 95 ± 0.99 µg/mL, SI: 6.10). A. reptans demonstrated antiproliferative potential against prostate and lung cancer cells. Therefore, additional investigations are needed to study the mechanism of the cytotoxicity for A. reptans.
dc.identifier.citationÇaşkurlu, A., Karavuş, Ş. N., Biltekin, S. N., Helvacı, E. Z. ve Karadağ, A. E. (2023). In vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts. Journal of Research in Pharmacy, 27(5), 2115-2123. https://doi.org/10.29228/jrp.2022.00
dc.identifier.doi10.29228/jrp.2022.00
dc.identifier.endpage2123
dc.identifier.issn2630-6344
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85171836463
dc.identifier.scopusqualityQ3
dc.identifier.startpage2115
dc.identifier.urihttps://doi.org/10.29228/jrp.2022.00
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11511
dc.identifier.volume27
dc.indekslendigikaynakScopus
dc.institutionauthorÇaşkurlu, Ayşegül
dc.institutionauthorKaravuş, Şule Nur
dc.institutionauthorBiltekin, Sevde Nur
dc.institutionauthorHelvacı, Esra Zeynep
dc.institutionauthorKaradağ, Ayşe Esra
dc.language.isoen
dc.publisherMarmara University
dc.relation.ispartofJournal of Research in Pharmacyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAjuga
dc.subjectCytotoxicity
dc.subjectHPLC
dc.subjectLamiaceae
dc.titleIn vitro cytotoxicity evaluation and phytochemical analysis of Ajuga reptans L. extracts
dc.typeArticle

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