Chemogenetic tools and genetic biosensors in redox biology: Probing Hyper7 signals in specific cellular locales

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dc.authorid0000-0002-9373-0808
dc.contributor.authorAltun, Hamza Yusuf
dc.contributor.authorErdoğan, Yusuf Ceyhun
dc.contributor.authorSeçilmiş, Melike
dc.contributor.authorEroğlu, Emrah
dc.date.accessioned2023-06-16T08:52:07Z
dc.date.available2023-06-16T08:52:07Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractH2O2 is one of the most studied reactive oxygen species (ROS) in redox biology because of its critical role in oxidant-dependent signaling pathways. Conventional approaches apply exogenous H2O2 on cells and tissues, which barely recapitulates intracellular redox signaling events. An alternative approach is D-amino acid oxidase (DAAO)-based chemogenetic tools that permit intracellular H2O2 generation by D-amino acid catalysis. Exploiting the targetability of the DAAOs to cellular ultralocales allow precise manipulation of the redox tone with high spatial and temporal resolution. DAAOs have been utilized in several redox studies, yet due to the incomplete characterization and the lack of fine-tuned protocol on how to employ DAAOs, the potential of this multiparametric imaging technology could not be fully exploited. Using the ultra-sensitive genetically encoded H2O2 biosensor (HyPer), we tested the performance of a modified version of DAAO (mDAAO) under hypoxic and hyperoxic conditions. Our multiparametric imaging approach unveiled that cell type, cellular locales, pericellular oxygen concentrations, D-amino acid type, and its concentrations are critical and modulatable factors that permit fine-tuned H2O2 generation with high precision. These findings and fine-tuned protocols provide practical approaches for redox scientists to spatiotemporally control oxidative stress-dependent signaling pathways in different cultured cell model systems.
dc.identifier.citationAltun, H. Y., Erdoğan, Y. C., Seçilmiş, M. ve Eroğlu, E. (2023). Chemogenetic tools and genetic biosensors in redox biology: Probing Hyper7 signals in specific cellular locales. 1st International Conference on the Future of Redox Biology içinde (S1-S1. ss.). Italy, 17-19 June 2022. https://doi.org/10.1016/j.freeradbiomed.2022.12.017
dc.identifier.doi10.1016/j.freeradbiomed.2022.12.017
dc.identifier.endpageS1
dc.identifier.issn0891-5849
dc.identifier.issn1873-4596
dc.identifier.issueSupplement: 1
dc.identifier.startpageS1
dc.identifier.urihttps://doi.org/10.1016/j.freeradbiomed.2022.12.017
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11098
dc.identifier.volume198
dc.identifier.wos000994915800003en_US
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.institutionauthorEroğlu, Emrah
dc.language.isoen
dc.publisherElsevier Science Inc.
dc.relation.ispartof1st International Conference on the Future of Redox Biologyen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectHydrogen Peroxide
dc.subjectChemogenetics
dc.subjectGenetically Encoded Biosensors
dc.titleChemogenetic tools and genetic biosensors in redox biology: Probing Hyper7 signals in specific cellular locales
dc.typeConference Object

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