Quantitative real-time PCR method to evaluate gene expression in zebrafish embryos

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dc.authorid0000-0002-6274-801X
dc.contributor.authorBeler, Merih
dc.contributor.authorCansız, Derya
dc.contributor.authorÜnal, İsmail
dc.contributor.authorEmekli Alturfan, Ebru
dc.date.accessioned2024-02-21T08:43:45Z
dc.date.available2024-02-21T08:43:45Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractThe fast zebrafish embryonic development offers an opportunity for the study of genes crucial for developmental processes. Several genes associated with human diseases have orthologs in zebrafish. Enhanced comprehension of a gene’s function can be achieved by knowing when and where it is expressed. This knowledge also makes it possible to alter a gene-by-gene knockdown in a time- and place-specific manner. Moreover, gene expression analyses contribute greatly to teratogenicity studies in zebrafish embryos. Therefore, the importance of examining the differences between the expressions of these genes has increased day by day. The incorporation of reverse transcription (RT) as the initial step prior to thermal cycling in quantitative real-time polymerase chain reaction (RT-qPCR) has made a significant contribution to RNA research. RT-qPCR is the gold standard and an effective method for gene expression analysis. Quick readout, high sensitivity, reproducibility, as well as high potential throughput, along with reliable quantification, are just a few advantages of RT-qPCR. However, there are drawbacks to its application, such as RNA’s inherent variability, impurities during RNA extraction, and variations in reverse transcription and PCR efficiencies. Implementing a precise normalization technique is crucial to account for these inaccuracies. In this chapter, the protocol for gene expression analysis by RT-qPCR for zebrafish embryos is explained.
dc.identifier.citationBeler, M., Cansız, D., Ünal, İ. ve Emekli Alturfan, E. (2024). Quantitative real-time PCR method to evaluate gene expression in zebrafish embryos. Methods in Molecular Biology içinde (553-561. ss.). Humana Press Inc. https://dx.doi.org/10.1007/978-1-0716-3625-1_36
dc.identifier.doi10.1007/978-1-0716-3625-1_36
dc.identifier.endpage561
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.pmid38285367
dc.identifier.scopus2-s2.0-85183729406
dc.identifier.scopusqualityQ3
dc.identifier.startpage553
dc.identifier.urihttps://dx.doi.org/10.1007/978-1-0716-3625-1_36
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12295
dc.identifier.volume2753
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCansız, Derya
dc.language.isoen
dc.publisherHumana Press Inc.
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararası
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGene Expression
dc.subjectQuantitative Real-Time Polymerase Chain Reaction
dc.subjectRNA
dc.subjectZebrafish Embryos
dc.titleQuantitative real-time PCR method to evaluate gene expression in zebrafish embryos
dc.typeBook Chapter

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