Sambulin A and B, non-glycosidic iridoids from Sambucus ebulus, exert significant in vitro anti-inflammatory activity in LPS-induced RAW 264.7 macrophages via inhibition of MAPKs's phosphorylation

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dc.authorid0000-0002-7921-8621
dc.contributor.authorAtay Balkan, İrem
dc.contributor.authorİlter Akülke, Ayça Zeynep
dc.contributor.authorBağatur, Yeşim
dc.contributor.authorTelci, Dilek
dc.contributor.authorGören, Ahmet Ceyhan
dc.contributor.authorKırmızıbekmez, Hasan
dc.contributor.authorYeşilada, Erdem
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:58:06Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:58:06Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Farmakognozi Ana Bilim Dalı
dc.descriptionWOS: 000406564500035
dc.descriptionPubMed ID: 28606808
dc.description.abstractEthnopharmacological relevance: The leaves of Sambucus ebulus L. (Adoxaceae) are widely used in Turkish folk medicine particularly against inflammatory disorders. The fresh leaves after wilted over fire or the poultices prepared are directly applied externally to heal burns, edema, eczema, urticarial and abscess. Two iridoids were recently isolated (sambulin A, sambulin B) from the leaves of S. ebulus. Aim of the study: This study aims to investigate the in vitro anti-inflammatory activities of these iridoids on LPS-induced RAW 264.7 macrophages. Materials and methods: Raw 264.7 macrophages were treated with 12.5, 25 and 50 mu g/ml Sambulin A and 6.25, 12.5 and 25 mu g/ml Sambulin B and induced with 1 mu g/ml lipopolysaccaharides (LPS). Effect of the compounds on nitric oxide (NO) production and cytoldnes (TNF alpha, IL-6) were determined by Griess and ELISA assays respectively. iNOS and the phosphorylation levels of MAPKs (ERK, JNK) were examined by Western Blot. Results: Sambulin A and sambulin B inhibited 52.82% and 72.88% of NO production at 50 and 25 mu g/ml concentrations respectively. The levels of iNOS were significantly decreased by both molecules, sambulin B at 25 mu g/ml almost completely decreased iNOS levels (97.53%). Both molecules significantly inhibited TNF alpha productions. However, only sambulin B inhibited IL-6 production. Consequently, it was shown that sambulin B exerted its effect through the inhibition of ERK and JNK phosphorylations. Conclusion: The prominent bioactivities exerted by two iridoids will contribute to explanation of the usage of S. ebulus in traditional medicine against rheumatoid diseases.
dc.description.sponsorshipScientific and Technological Research Council of Turkey [TUBITAK] [110S197]en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey [TUBITAK Project no: 110S197].en_US
dc.identifier.citationAtay Balkan, İ., İlter Alkülke, A. Z., Bağatur, Y., Telci, D., Gören, A. C., Kırmızıbekmez, H. ... Yeşilada, E. (2017). Sambulin A and B, non-glycosidic iridoids from Sambucus ebulus, exert significant in vitro anti-inflammatory activity in LPS-induced RAW 264.7 macrophages via inhibition of MAPKs's phosphorylation. Journal of Ethnopharmacology, 206, 347-352. https://dx.doi.org/10.1016/j.jep.2017.06.002
dc.identifier.doi10.1016/j.jep.2017.06.002
dc.identifier.endpage352
dc.identifier.issn0378-8741
dc.identifier.scopusqualityQ1
dc.identifier.startpage347
dc.identifier.urihttps://dx.doi.org/10.1016/j.jep.2017.06.002
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3108
dc.identifier.volume206
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Irelan Ltd
dc.relation.ecinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/110S197
dc.relation.ispartofJournal of Ethnopharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSambucus Ebulus
dc.subjectIridoids
dc.subjectSambulin A and B
dc.subjectInducible Nitric Oxide Synthase
dc.subjectMitogen Activated Protein Kinases
dc.subjectCytokines
dc.titleSambulin A and B, non-glycosidic iridoids from Sambucus ebulus, exert significant in vitro anti-inflammatory activity in LPS-induced RAW 264.7 macrophages via inhibition of MAPKs's phosphorylation
dc.typeArticle

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