Alcian blue staining for chondrocranium development in zebrafish

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dc.authorid0000-0002-6274-801X
dc.contributor.authorBeler, Merih
dc.contributor.authorÜnal, İsmail
dc.contributor.authorCansız, Derya
dc.contributor.authorEmekli Alturfan, Ebru
dc.date.accessioned2024-02-21T07:57:04Z
dc.date.available2024-02-21T07:57:04Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractCraniofacial abnormalities are one of the most frequent birth malformations in humans, affecting around one in every thousand live births. The zebrafish (Danio rerio), a model organism that has seen increased usage in toxicological research in recent years, is ideal for assessing the effects of various chemicals on bone and cartilage structures. Chondrogenesis developed in zebrafish embryos by embryonic day 2, and supporting cartilage components are apparent at hatching (72 h post-fertilization). Individual cartilage may be observed using Alcian Blue staining as early as 2 days post-fertilization (dpf). The preferential binding of Alcian Blue causes the staining of zebrafish cartilage to acidic glycoproteins in an acidic solution (pH 2.2). In 72–120 hpf embryos, the cranial skeleton is easily visible after cartilage staining using Alcian Blue. Various cranial lengths and structures can be determined by measuring specific distances and angles to optimize the quantitative analysis of cranial malformations in zebrafish after exposure to various toxic agents. This chapter explains the Alcian Blue staining procedure to identify craniofacial cartilaginous structures in zebrafish embryos.
dc.identifier.citationBeler, M., Ünal, İ., Cansız, D. ve Emekli Alturfan, E. (2024). Alcian blue staining for chondrocranium development in zebrafish. Methods in Molecular Biology içinde (447-457. ss.). Humana Press Inc. https://dx.doi.org/10.1007/978-1-0716-3625-1_27
dc.identifier.doi10.1007/978-1-0716-3625-1_27
dc.identifier.endpage457
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.pmid38285358
dc.identifier.scopus2-s2.0-85183739989
dc.identifier.scopusqualityQ3
dc.identifier.startpage447
dc.identifier.urihttps://dx.doi.org/10.1007/978-1-0716-3625-1_27
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12294
dc.identifier.volume2753
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCansız, Derya
dc.language.isoen
dc.publisherHumana Press Inc.
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararası
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAlcian Blue
dc.subjectCraniofacial Abnormalities
dc.subjectStaining Methods
dc.subjectTeratogenicity
dc.subjectZebrafish Embryo
dc.titleAlcian blue staining for chondrocranium development in zebrafish
dc.typeBook Chapter

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