Are specific gene expressions of extracellular matrix and nucleus pulposus affected by primary cell cultures prepared from intact or degenerative intervertebral disc tissues?

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dc.authorid0000-0003-2003-6337
dc.authorid0000-0002-8084-7855
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorKaplan, Necati
dc.contributor.authorAkyuva, Yener
dc.contributor.authorGönültaş, Aylin
dc.contributor.authorAteş, Özkan
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:49:54Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:49:54Z
dc.date.issued2019
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Farmakoloji Ana Bilim Dalı
dc.descriptionWOS: 000454722700007
dc.descriptionPubMed ID: 29484626
dc.description.abstractAIM: To determine the gene expression patterns of nucleus pulposus (NP) in cell cultures obtained from degenerated or intact tissues. MATERIAL and METHODS: Whereas 12 of the cases were diagnosed with lumbar disc herniation and had undergone lumbar microdiscectomy, 12 cases had undergone traumatic intervertebral discectomy and corpectomy, along with discectomy after spinal trauma. NP-specific markers and gene expressions of the reagents of the extracellular matrix in the experimental setup were tested at the 0th, 24th, and 48th hours by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Visual evaluations were simultaneously made in all samples using invert and fluorescence microscopy. Vitality and proliferation analyses were evaluated by UV spectrophotometer. As a method of statistical evaluation, Spearman was used for categorical variants, and the Pearson correlation was used for variants with numerical and plain distribution. RESULTS: No association was found either between the tissue type and times (r=0.000; p=1.000) or between the region that the tissue was obtained from and hypoxia transcription factor-1 alpha (HIF-1 alpha) gene expression (r=0.098; p=0.245). There was no correlation between cell proliferation and chondroadherin (CHAD) expression or between type II collagen (COL2A1) and CHAD gene expressions. It was found that CHAD and HIF-1 alpha gene expressions and HIF-1 alpha and COL2A1 gene expressions affected cell proliferation. CONCLUSION: Cell culture setups are of paramount importance because they may influence the pattern of changes in the gene expressions of the cells used in these setups.
dc.identifier.citationKaraaslan, N., Yılmaz, İ., Özbek, H., Yaşar Şirin, D., Kaplan, N., Akyuva, Y. … Ateş, Ö. (2019). Are specific gene expressions of extracellular matrix and nucleus pulposus affected by primary cell cultures prepared from intact or degenerative intervertebral disc tissues? Turkish Neurosurgery, 29(1), 43-52. https://dx.doi.org/10.5137/1019-5149.JTN.22210-17.2
dc.identifier.doi10.5137/1019-5149.JTN.22210-17.2
dc.identifier.endpage52
dc.identifier.issn1019-5149
dc.identifier.issue1
dc.identifier.scopusqualityQ3
dc.identifier.startpage43
dc.identifier.urihttps://dx.doi.org/10.5137/1019-5149.JTN.22210-17.2
dc.identifier.urihttps://hdl.handle.net/20.500.12511/1812
dc.identifier.volume29
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Neurosurgical Society
dc.relation.ispartofTurkish Neurosurgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnnulus Fibrosus
dc.subjectDegenerative Disc Disease
dc.subjectNucleus Pulposus
dc.subjectSpecific Marker
dc.subjectPharmaco-Molecular Therapy
dc.subjectHuman Primary Cell Culture
dc.titleAre specific gene expressions of extracellular matrix and nucleus pulposus affected by primary cell cultures prepared from intact or degenerative intervertebral disc tissues?
dc.typeArticle

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