Investigation of changes in liver microanatomy in the steatosis model created by permanent canula in rats

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dc.authorid0000-0002-5539-2342
dc.contributor.authorNalbant, Asrin
dc.contributor.authorŞakul, Bayram Ufuk
dc.contributor.authorYücel, Ferruh
dc.date.accessioned2022-10-26T08:45:19Z
dc.date.available2022-10-26T08:45:19Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Anatomi Ana Bilim Dalı
dc.description.abstractObjective: The knowledge of nonalcoholic fatty liver disease (NAFLD) and Nonalcoholic Steatohepatitis (NASH) is limited to the findings from available suitable models for this disease. A number of rodent models have been described in which relevant liver pathology develops in an appropriate metabolic context. In this experimental study, it was aimed to create a new liver fat model by giving fat from the portal vein of rats and to visualize the changes in the liver with advanced microscopic techniques.Methods: 28 female rats were used in the study. Permanent intraabdominal cannulas were inserted into the portal vein of the rats. Rats were randomly divided four group. Intralipid 20% substance was injected through cannula to the experimental groups during the test period. Control group received saline at the same rate. At the end of the experiment, the animals were visualized with a laser speckle microscope and livers were divided into sections according to the stereological method. The sections were painted with Hematoxylin-Eosin, Oil red o, Masson trichoma, Bodipy, Nile red. Sections were evaluated under a microscope.Results: Ballooning, inflammation and fibrosis were observed in the 2 week intralipid group. In the 1 week intralipid group, the rate of parenchyma decreased while the sinusoid rate increased, and sinusoid rate increased significantly in the 2 week intralipid (p<0.05). Conclusion: According to the findings, steatohepatitis was detected in the 2 week intralipid, whereas only steatosis was observed in the 1 week intralipid. Thus, it was concluded that the newly formed rat model causes steatosis.
dc.identifier.citationNalbant, A., Şakul, B. U. ve Yücel, F. (2022). Investigation of changes in liver microanatomy in the steatosis model created by permanent canula in rats. Clinical and Experimental Health Sciences, 12(3), 659-664. https://doi.org/10.33808/clinexphealthsci.948391
dc.identifier.doi10.33808/clinexphealthsci.948391
dc.identifier.endpage664
dc.identifier.issn2459-1459
dc.identifier.issue3
dc.identifier.startpage659
dc.identifier.trdizinid1127937
dc.identifier.urihttps://doi.org/10.33808/clinexphealthsci.948391
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9882
dc.identifier.volume12
dc.identifier.wos000863981200017en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorŞakul, Bayram Ufuk
dc.language.isoen
dc.publisherMarmara University
dc.relation.ispartofClinical and Experimental Health Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectNAFLD
dc.subjectSteatosis
dc.subjectPortal Vein
dc.subjectConfocal Microscope
dc.subjectLaser Speckle
dc.titleInvestigation of changes in liver microanatomy in the steatosis model created by permanent canula in rats
dc.typeArticle

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