Chitosan modification-enhanced silencing effect of Ad5-shPDGF-D vector in breast cancer cell line MDA-MB-231

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dc.authorid0000-0002-1693-9579
dc.contributor.authorEkentok Atıcı, Ceyda
dc.contributor.authorAkbuğa, Jülide
dc.date.accessioned2023-05-30T07:47:58Z
dc.date.available2023-05-30T07:47:58Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Eczacılık Teknolojisi Bölümü, Farmasötik Teknoloji Ana Bilim Dalı
dc.description.abstractBackground: Gene therapeutics are being developed to treat metastatic breast tumors, which are mostly resistant to conventional therapies. Targeting platelet-derived growth factor-D (PDGF-D) is a viable approach because it is known to play roles in angiogenesis and tumor growth. The success of gene therapy is largely dependent on delivery vectors, but both viral and nonviral delivery vectors have their disadvantages. Evolving hybrid vectors are being used to overcome those disadvantages. Objectives: In this study, we aimed to prepare a recombinant adenovirus type-5 (Ad5)/chitosan hybrid vector to deliver shPDGF-D in a breast cancer cell line by the noncovalent coating of the Ad5 surface with chitosan, a natural polymer. Methods: The Ad5/chitosan hybrid vector was prepared by the noncovalent coating of the Ad5 surface with different molecular weights (low and high) and different amounts of chitosan (12.5, 25, and 50 µg), and the effect of silencing PDGF-D was investigated in the MDA-MB-231 cell line. Results: In vitro characterization studies showed that the noncovalent chitosan coating increased the size of the Ad5 particle and changed the surface charge from-16.53 mV to slightly neutral. In vitro cell culture studies also showed that the addition of chitosan with both low (73.61%) and high (65.86%) molecular weight increased the PDGF-D silencing efficiency of the Ad5 vector (42.44%) at 48 hours. While low-molecular-weight chitosan had faster effects, high-molecular-weight chitosan provided a more sustained effect in PDGF-D silencing. Conclusion: The results indicate that noncovalent chitosan modification may improve the therapeutic effects of the Ad5 vector, offering the potential for further in vitro and in vivo experiments.
dc.description.sponsorshipMedipol University ; Marmara Universityen_US
dc.identifier.citationEkentok Atıcı, C. ve Akbuğa, J. (2023). Chitosan modification-enhanced silencing effect of Ad5-shPDGF-D vector in breast cancer cell line MDA-MB-231. Current Drug Delivery, 20(8), 1176-1187. https://doi.org/10.2174/1567201819666220429093821
dc.identifier.doi10.2174/1567201819666220429093821
dc.identifier.endpage1187
dc.identifier.issn1567-2018
dc.identifier.issn1875-5704
dc.identifier.issue8
dc.identifier.pmid35507787
dc.identifier.scopus2-s2.0-85158938249
dc.identifier.scopusqualityQ2
dc.identifier.startpage1176
dc.identifier.urihttps://doi.org/10.2174/1567201819666220429093821
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10993
dc.identifier.volume20
dc.identifier.wos001004684700010en_US
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAkbuğa, Jülide
dc.language.isoen
dc.publisherBentham Science Publishers
dc.relation.ispartofCurrent Drug Deliveryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAd5
dc.subjectChitosan
dc.subjectPDGF-D
dc.subjectBreast Cancer
dc.subjectGene Silencing
dc.subjectHybrid Vector
dc.titleChitosan modification-enhanced silencing effect of Ad5-shPDGF-D vector in breast cancer cell line MDA-MB-231
dc.typeArticle

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