Traditional herbal drugs against liver diseases – Experimented in vitro using HepG2 cells for induced steatosis

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dc.authorid0000-0002-3412-0807
dc.contributor.authorKaradağ, Ayşe Esra
dc.contributor.authorOkur, Mehmet Evren
dc.contributor.authorÖzhan, Yağmur
dc.contributor.authorSipahi, Hande
dc.contributor.authorDemirci, Fatih
dc.date.accessioned2023-10-03T06:24:57Z
dc.date.available2023-10-03T06:24:57Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Farmakognozi Ana Bilim Dalı
dc.description.abstractSalvia fruticosa leaves, Malva sylvestris flowers, Taraxacum officinale aerial parts, Plantago ovata seeds, Tanacetum parthenium aerial parts, and Allium sativum bulbs are documented for traditional use against hepatic disorders and different liver diseases. To evaluate herbal drug material for potential use against liver diseases, at molecular level for the efficacy linked to ethnobotanical documented data.Different herbal extracts were prepared and standardized by HPLC, according to European Pharmacopoeia. Initially 0.25 mg/mL each standardized extract was applied to oleic+palmitic acid induced fatty liver using a HepG2 cell culture model. ALT, AST, GSH, and MDA levels were comparatively analyzed, in addition to cell Nil Red staining. The highest activity for MDA reduction was observed for the A. sativum extract at 48.2% level, followed by 36.4% for M. sylvestris, and S. fruticosa extracts with 27.3% reduction, respectively. Glutathione levels increased to 59.1% when A. sativum extract was applied. M. sylvestris extract increased the glutathione levels in the medium by 49.7%; S. fruticosa extract decreased ALT levels by 53.5% and M. sylvestris extract by 38.5%, whereas the standard resveratrol reduced ALT level by 30.9%, respectively. The AST levels for M. sylvestris extract was 46.5%, compared to resveratrol by 93%. A. sativum, M. sylvestris, and S. fruticosa standard extracts showed relatively good correlation and activity where further in vivo studies should be performed.
dc.identifier.citationKaradağ, A. E., Okur, M. E., Özhan, Y., Sipahi, H. ve Demirci, F. (2023). Traditional herbal drugs against liver diseases – Experimented in vitro using HepG2 cells for induced steatosis. Journal of Research in Pharmacy, 27(5), 1831-1847. https://doi.org/10.29228/jrp.467
dc.identifier.doi10.29228/jrp.467
dc.identifier.endpage1847
dc.identifier.issn2630-6344
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85171540228
dc.identifier.scopusqualityQ3
dc.identifier.startpage1831
dc.identifier.urihttps://doi.org/10.29228/jrp.467
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11516
dc.identifier.volume27
dc.identifier.wos001069183100010en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKaradağ, Ayşe Esra
dc.language.isoen
dc.publisherMarmara University
dc.relation.ispartofJournal of Research in Pharmacyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/221S351
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectEthnobotany
dc.subjectHPLC
dc.subjectFatty Liver
dc.subjectCell Culture
dc.titleTraditional herbal drugs against liver diseases – Experimented in vitro using HepG2 cells for induced steatosis
dc.typeArticle

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