Transformation of acinetobacter baumannii: Electroporation

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dc.authorid0000-0002-2752-1223
dc.contributor.authorThompson, Mitchell Greg
dc.contributor.authorYıldırım, Süleyman
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:35:46Z
dc.date.available10.07.201910:49:14
dc.date.available2019-07-10T19:35:46Z
dc.date.issued2019
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractAlthough the pan and the core genome of Acinetobacter baumannii and its essential genes are relatively well characterized, functional characterization of these genes has not paralleled the genome-level studies. However, recently developed genetic tools and optimized protocols are poised to accelerate genetic manipulation of A. baumannii. Transferring exogenous DNA into the cytosol of bacteria cells is a critical step in genetic characterizations. Conjugation is restricted to the transfer of DNA from one bacterial cell to another, and only a portion of A. baumannii clinical isolates are naturally competent. Electroporation, which is thought to transiently create aqueous pores in the membrane, is a preferred method in transferring exogenous DNA as it does not have such limitations. Several factors contribute to efficiency of electroporation and often need to be empirically optimized to maximize efficiency of this procedure. Here we provide an optimized electroporation protocol and guidance for electroporation of clinical MDR isolates of A. baumannii.
dc.identifier.citationThompson, M. G. ve Yıldırım, S. (2019). Transformation of acinetobacter baumannii: Electroporation. Methods in Molecular Biology içinde (69-74. ss.). Humana Press Inc. https://dx.doi.org/10.1007/978-1-4939-9118-1_7
dc.identifier.doi10.1007/978-1-4939-9118-1_7
dc.identifier.endpage74
dc.identifier.issn1064-3745
dc.identifier.scopusqualityQ3
dc.identifier.startpage69
dc.identifier.urihttps://hdl.handle.net/20.500.12511/938
dc.identifier.urihttps://dx.doi.org/10.1007/978-1-4939-9118-1_7
dc.identifier.volume1946
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHumana Press Inc.
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararası
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcinetobacter Baumannii
dc.subjectElectroporation
dc.subjectMultidrug Resistant (MDR)
dc.subjectTransformation
dc.titleTransformation of acinetobacter baumannii: Electroporation
dc.typeBook Chapter

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