Microarray analysis to determine gene expression changes in zebrafish embryos

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dc.authorid0000-0002-6274-801X
dc.contributor.authorÜnal, İsmail
dc.contributor.authorBeler, Merih
dc.contributor.authorCansız, Derya
dc.contributor.authorEmekli Alturfan, Ebru
dc.date.accessioned2024-02-20T08:36:00Z
dc.date.available2024-02-20T08:36:00Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractChemical exposure in humans begins from the zygote stage and continues throughout the development of the embryo and the fetus. Zebrafish are one of the most powerful model organisms used in many research areas, including genetics, environmental toxicology, development, DNA damage and repair, cancer, and other diseases. Among the advantages that facilitate the use of zebrafish as a model for studies are features such as high homology with the human genome, small size, and high reproductive potential in short periods. The use of zebrafish embryos in research has increased rapidly due to their advantageous properties, including extrauterine development and the transparent feature of the embryos. However, there are thousands of genes that can be encountered in research, and in this case, the workforce is too much. This workload has been alleviated with the developed technologies. Microarray is one of these technologies. An important parameter in this assay is the RIN value. The RIN value ranges from 1 to 10, indicating mRNA degradation, and therefore helps to decide whether to continue the study. In this chapter, microarray analysis, which is one of the main techniques used in the determination of gene expression in zebrafish embryos, is described.
dc.identifier.citationÜnal, İ., Beler, M., Cansız, D. ve Emekli Alturfan, E. (2024). Microarray analysis to determine gene expression changes in zebrafish embryos. Methods in Molecular Biology içinde (563-582. ss.). Humana Press Inc. https://dx.doi.org/10.1007/978-1-0716-3625-1_37
dc.identifier.doi10.1007/978-1-0716-3625-1_37
dc.identifier.endpage582
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.pmid38285368
dc.identifier.scopus2-s2.0-85184344740
dc.identifier.scopusqualityQ3
dc.identifier.startpage563
dc.identifier.urihttps://dx.doi.org/10.1007/978-1-0716-3625-1_37
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12290
dc.identifier.volume2753
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCansız, Derya
dc.language.isoen
dc.publisherHumana Press Inc.
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararası
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcRNA
dc.subjectMicroarray Analysis
dc.subjectRIN Values
dc.subjectZebrafish Embryo
dc.titleMicroarray analysis to determine gene expression changes in zebrafish embryos
dc.typeBook Chapter

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