Development, characterization of naringenin-loaded promising microemulsion formulations, and demonstration of anti-aging efficacy by in vitro enzyme activity and gene expression

Basit Öğe Kaydı
dc.authorid0000-0002-3412-0807
dc.contributor.authorŞahin, Dilara
dc.contributor.authorÇağlar, Emre Şefik
dc.contributor.authorBoran, Tuğçe
dc.contributor.authorKaradağ, Ayşe Esra
dc.contributor.authorÖzhan, Gül
dc.contributor.authorÜstündağ Okur, Neslihan
dc.date.accessioned2023-04-28T06:45:28Z
dc.date.available2023-04-28T06:45:28Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Farmakognozi Ana Bilim Dalı
dc.description.abstractThis study aims to produce microemulsion formulations containing naringenin for anti-aging reasons. For this purpose, formulations of topical microemulsions including different cosurfactants and surfactants were developed, and a triangle phase diagram was utilized to determine the optimal microemulsion system. On the developed microemulsion formulations, characterization studies were performed. Ex vivo permeation-penetrating and in vitro release experiments were conducted. Anti-elastase, anti-collagenase, anti-tyrosinase, and antioxidant properties of the formulations were also evaluated. Experiments were conducted on cytotoxicity, cellular ROS, gene expression levels of oxidative stress-related genes, and cell culture. Droplet size, polydispersity index, zeta potential, pH, viscosity, turbidity of naringenin loaded formulations were found between 0.9147 ± 0.0316 and 1.938 ± 0.085 nm, 0.373 ± 0.025 and 0.410 ± 0.019, ?0.0517 ± 0.0138 and ?0.0990 ± 0.0110 mV, 5.110 ± 0.006 and 5.530 ± 0.006, 163.33 ± 5.77 and 450.00 ± 0.00 cP, 5.723 ± 0.068 and 9.323 ± 0.264 NTU, respectively. At the end of 24 h, the in vitro release research shows that more than 50% of naringenin has been released. Elastase and collagenase enzyme inhibitions of naringenin loaded formulations were found between 31.98 ± 0.15 and 41.74 ± 0.09%, 39.99 ± 0.19 and 47.78 ± 0.04%, respectively. Furthermore, NRF-2 and HO-1 gene expression levels did not show a significant change in the cells exposed to only naringenin loaded formulations. To conclude, developed microemulsions seem to be promising formulations that can be used for anti-aging purposes.
dc.description.sponsorshipUniversity of Health Sciences Lahoreen_US
dc.identifier.citationŞahin, D., Çağlar, E. Ş., Boran, T., Karadağ, A. E., Özhan, G. ve Üstündağ Okur, N. (2023). Development, characterization of naringenin-loaded promising microemulsion formulations, and demonstration of anti-aging efficacy by in vitro enzyme activity and gene expression. Journal of Drug Delivery Science and Technology, 84. https://dx.doi.org/10.1016/j.jddst.2023.104422
dc.identifier.doi10.1016/j.jddst.2023.104422
dc.identifier.issn1773-2247
dc.identifier.scopus2-s2.0-85152373064
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://dx.doi.org/10.1016/j.jddst.2023.104422
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10902
dc.identifier.volume84
dc.identifier.wos000984986700001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKaradağ, Ayşe Esra
dc.language.isoen
dc.publisherEditions de Sante
dc.relation.ispartofJournal of Drug Delivery Science and Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAnti-Elastase
dc.subjectAntiaging
dc.subjectCell Culture
dc.subjectGene Expressions
dc.subjectMicroemulsion
dc.subjectNaringenin
dc.titleDevelopment, characterization of naringenin-loaded promising microemulsion formulations, and demonstration of anti-aging efficacy by in vitro enzyme activity and gene expression
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
Karadag-Ayse-2023.pdf
Boyut:
2.55 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Eczacılık Fakültesi Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu