In vitro activities of colistin, tigecycline and tobramycin, alone or in combination, against carbapenem-resistant enterobacteriaceae strains

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dc.authorid0000-0001-8022-7325
dc.contributor.authorÖzbek, Berna
dc.contributor.authorMataracı Kara, Emel
dc.contributor.authorEr, Sevda
dc.contributor.authorÖzdamar, Melda
dc.contributor.authorYılmaz, Mesut
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:58:13Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:58:13Z
dc.date.issued2015
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Ana Bilim Dalı
dc.descriptionWOS: 000366948100009
dc.descriptionPubMed ID: 27842873
dc.description.abstractThe aim of this study was to investigate the activities of various antibiotics, alone or in combination, against carbapenem-resistant Enterobacteriaceae (CRE). Minimum inhibitory concentrations (MICs) of meropenem, colistin, tigecycline and tobramycin were determined by microbroth dilution against 40 clinical strains. Carbapenemase-encoding genes were detected by PCR using specific primers. The in vitro synergistic activities of tigecycline, colistin and tobramycin in double antibiotic combinations were determined by the microbroth chequerboard technique, and results were interpreted using the fractional inhibitory concentration index (FICI). To confirm the results acquired by the chequerboard method, time-kill assays were performed on eight isolates representing four different susceptibility patterns. Based on MIC90 values, colistin was the most potent agent, followed by tigecycline and tobramycin. According to PCR studies, carbapenem resistance in tested Enterobacteriaceae isolates was most often mediated by OXA-48-type carbapenemases. With an FICI of <= 0.5 as the cut-off, synergistic interactions were most frequent with tigecycline + tobramycin (30%); other results for synergistic interactions were 23% for colistin + tobramycin and 9% for colistin + tigecycline. By time-kill assays, all tested antibiotic combinations demonstrated synergistic activity against at least three of the eight strains at 1x or 4x MIC. Overall, the combinations used in this study were effective regimens, demonstrating synergy or no interaction (indifference) against all tested strains. No antagonism was observed with either of the techniques. The findings of this study might play a useful role in selecting appropriate combinations when a single agent is inadequate against CRE strains.
dc.description.sponsorshipResearch Fund of The University of Istanbul (Istanbul, Turkey) [4703]en_US
dc.description.sponsorshipThis work was supported by a grant from the Research Fund of The University of Istanbul (Istanbul, Turkey) [Project no. 4703].en_US
dc.identifier.citationÖzbek, B., Mataracı Kara, E., Er, S., Özdamar, M. ve Yılmaz, M. (2015). In vitro activities of colistin, tigecycline and tobramycin, alone or in combination, against carbapenem-resistant Enterobacteriaceae strains. Journal of Global Antimicrobial Resistance, 3(4), 278-282. https://dx.doi.org/10.1016/j.jgar.2015.09.001
dc.identifier.doi10.1016/j.jgar.2015.09.001
dc.identifier.endpage282
dc.identifier.issn2213-7165
dc.identifier.issn2213-7173
dc.identifier.issue4
dc.identifier.scopusqualityQ3
dc.identifier.startpage278
dc.identifier.urihttps://dx.doi.org/10.1016/j.jgar.2015.09.001
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3129
dc.identifier.volume3
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Global Antimicrobial Resistanceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAntibiotics
dc.subjectCombination
dc.subjectGram-negative
dc.subjectEnterobacteriaceae
dc.subjectResistance
dc.subjectESBL
dc.titleIn vitro activities of colistin, tigecycline and tobramycin, alone or in combination, against carbapenem-resistant enterobacteriaceae strains
dc.typeArticle

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