HPLC-FRAP methodology and biological activities of different stem bark extracts of Cajanus cajan (L.) Millsp
| dc.authorid | 0000-0002-4449-0537 | |
| dc.contributor.author | Sinan, Kouadio Ibrahime | |
| dc.contributor.author | Mahomoodally, Mohamad Fawzi | |
| dc.contributor.author | Eyüpoğlu, Ozan Emre | |
| dc.contributor.author | Etienne, Ouattara Katinan | |
| dc.contributor.author | Sadeer, Nabeelah Bibi | |
| dc.contributor.author | Ak, Güneş | |
| dc.contributor.author | Behl, Tapan | |
| dc.contributor.author | Zengin, Gökhan | |
| dc.date.accessioned | 2021-01-21T08:53:51Z | |
| dc.date.available | 2021-01-21T08:53:51Z | |
| dc.date.issued | 2021 | |
| dc.department | İstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümü, Biyokimya Ana Bilim Dalı | |
| dc.description.abstract | Cajanus cajan. (L.) Millsp. (C. cajan) (Family: Fabaceae) also known as pigeon pea, is a famous food and cover/forage crop bearing a high amount of key amino acids (methionine, lysine and tryptophan). This study investigated into the total phenolic (TPC), flavonoid content (TFC), antioxidant ]2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2 -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity, total antioxidant capacity (TAC) (phosphomolybdenum) and metal chelating] activities and enzyme [alpha-amylase, alpha-glucosidase, tyrosinase, acetyl-(AChE), butyry1-(BChE) cholinesterase] inhibitory effects of four extracts (methanol, hexane, ethyl acetate, aqueous) prepared from C. cajan stem bark. Direct identification of antioxidants was also conducted using the high performance liquid chromatography-ferric reducing antioxidant power (HPLC-FRAP) system. The highest TPC and TFC were recorded with the methanolic (23.22 +/- 0.17 mg GAE/g) and ethyl acetate extracts (19.43 +/- 0.24 mg RE/g), respectively. The methanolic extract exhibited important antioxidant activity with DPPH (38.41 +/- 0.05 mg Trolox equivalent (TE)/g), ABTS (70.49 +/- 3.62 mg TE/g), CUPRAC (81.86 +/- 2.40 mg TE/g), FRAP (42.96 +/- 0.59 mg TE/g) and metal chelating (17.00 +/- 1.26 mg ethylenediaminetetraacetic acid equivalent/g). p-coumaric and caffeic acid were the predominant antioxidants in the samples. Results from enzymatic assays showed the potential abilities of hexane extract in inhibiting the AChE, BChE, alpha-amylase and alpha-glucosidase enzymes. From the results obtained in this study, it can be concluded that C. cajan can be considered as a promising source of antioxidants and key enzyme inhibitors that can be exploited for future bioproduct development. | |
| dc.identifier.citation | Sinan, K. I., Mahomoodally, M. F., Eyüpoğlu, O. E., Etienne, O. K., Sadeer, N. B., Ak, G. ... Zengin, G. (2021). HPLC-FRAP methodology and biological activities of different stem bark extracts of Cajanus cajan (L.) Millsp. Journal of Pharmaceutical and Biomedical Analysis. Journal of Pharmaceutical and Biomedical Analysis, 192. https://dx.doi.org/10.1016/j.jpba.2020.113678 | |
| dc.identifier.doi | 10.1016/j.jpba.2020.113678 | |
| dc.identifier.issn | 0731-7085 | |
| dc.identifier.issn | 1873-264X | |
| dc.identifier.scopusquality | Q2 | |
| dc.identifier.uri | https://dx.doi.org/10.1016/j.jpba.2020.113678 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12511/6334 | |
| dc.identifier.volume | 192 | |
| dc.identifier.wosquality | Q2 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.relation.ispartof | Journal of Pharmaceutical and Biomedical Analysis | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | Antioxidants | |
| dc.subject | Bioactive Compounds | |
| dc.subject | Pigeon Pea | |
| dc.subject | Enzyme Inhibition | |
| dc.subject | Natural Products | |
| dc.title | HPLC-FRAP methodology and biological activities of different stem bark extracts of Cajanus cajan (L.) Millsp | |
| dc.type | Article |
