Identification of PDE10A related proteins via proteomic analysis
| dc.authorid | 0000-0002-9476-8488 | |
| dc.authorid | 0000-0002-4552-6394 | |
| dc.authorid | 0000-0002-5072-132X | |
| dc.authorid | 0000-0001-6494-8923 | |
| dc.contributor.author | Beker, Mustafa Çağlar | |
| dc.contributor.author | Yelkenci, Hayriye Ecem | |
| dc.contributor.author | Çağlayan, Berrak | |
| dc.contributor.author | Kılıç, Ertuğrul | |
| dc.date.accessioned | 2023-01-24T06:43:20Z | |
| dc.date.available | 2023-01-24T06:43:20Z | |
| dc.date.issued | 2022 | |
| dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı | |
| dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü | |
| dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER) | |
| dc.department | İstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Genetik Ana Bilim Dalı | |
| dc.description.abstract | Aim: Phosphodiesterase 10A (PDE10A) regulates the expression of secondary messengers of cyclic adenosine monophosphate and cyclic guanosine monophosphate, which control several intracellular signaling pathways. Recently, deactivation of PDE10A has been a notable target for the treatment of neurodegenerative diseases. Herein, we identified the effects of PDE10A inhibition on protein profile using TAK-063 under physiological condi- tions in mice. Materials and Methods: In this study, 8-12 weeks old male C57BL6/J mice were divided into vehicle or 3 mg/kg TAK-063 groups. Thirty minutes after oral delivery of vehicle or TAK-063, animals were sacrificed and liquid chromatography-mass spectrome- try/mass spectrometry (LC-MS/MS) mediated proteomic analyses were performed from tissue samples taken from the striatum region of mice. After the LC-MS/MS analysis, identified proteins were classified based on biological activity, molecular function, and signal transduction pathways using PANTHER (protein annotation through evolutionary relationship, http://www.pantherdb.org/) program. Results: As a result of proteomic analyses, 1873 different proteins were identified. Sixty- one different proteins changed significantly depending on the administration of TAK-063. According to PANTHER classification, a significant part of the identified proteins found to be in the metabolite interconversion enzyme, transporter, and protein modifying enzyme category. The molecular function classification includes the catalytic activity, transporter activity, and binding functions. The signal transduction pathway analysis demonstrated that PDE10A affects ATP synthesis, FGF signaling, EGF receptor signaling, Huntington’s Disease, Parkinson’s Disease, pyrimidine metabolism, and ubiquitin-proteasome signal transduction pathways. Conclusion: TAK-063 mediated PDE10 deactivation is an essential target in the mech- anism of energy metabolism and neurodegenerative diseases. | |
| dc.identifier.citation | Beker, M. Ç., Yelkenci, H. E., Çağlayan, B. ve Kılıç, E. (2022). Identification of PDE10A related proteins via proteomic analysis. Annals of Medical Research, 29(8), 894-899. https://dx.doi.org/10.5455/annalsmedres.2022.05.153 | |
| dc.identifier.doi | 10.5455/annalsmedres.2022.05.153 | |
| dc.identifier.endpage | 899 | |
| dc.identifier.issn | 2636-7688 | |
| dc.identifier.issue | 8 | |
| dc.identifier.startpage | 894 | |
| dc.identifier.trdizinid | 1126892 | |
| dc.identifier.uri | https://dx.doi.org/10.5455/annalsmedres.2022.05.153 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12511/10361 | |
| dc.identifier.volume | 29 | |
| dc.indekslendigikaynak | TR-Dizin | |
| dc.institutionauthor | Beker, Mustafa Çağlar | |
| dc.institutionauthor | Yelkenci, Hayriye Ecem | |
| dc.institutionauthor | Çağlayan, Berrak | |
| dc.institutionauthor | Kılıç, Ertuğrul | |
| dc.language.iso | en | |
| dc.publisher | İnönü University Faculty of Medicine | |
| dc.relation.ispartof | Annals of Medical Research | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.subject | Liquid Chromatography-Tandem Mass Spectrometry | |
| dc.subject | Phosphodiesterase 10A | |
| dc.subject | Proteomics | |
| dc.subject | TAK-063 | |
| dc.title | Identification of PDE10A related proteins via proteomic analysis | |
| dc.type | Article |
