Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements

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dc.contributor.authorErim, Ümit Can
dc.contributor.authorAyşit, Neşe
dc.date.accessioned2025-07-03T12:57:21Z
dc.date.available2025-07-03T12:57:21Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractQuercetin is a ubiquitous bioactive flavonoid found in broad selection of dietary supplements. It has potential beneficial health effects and induces therapeutic activity in many diseases including different types of cancer. Quercetin exerts cytotoxic activity in cancer and induces apoptotic cell death. Albeit, each dietary supplement consists of impurities, which in turn diminish the biological activity of the quercetin. Herein, we extracted quercetin from 5 different marketed dietary supplements in tablet formulation and detected their quercetin content by developing and validating an RP-HPLC method. We further investigated the cytotoxic activity of quercetin and its role in apoptosis through mitochondrial pathway in MCF-7 and 4T1 breast cancer cell lines. Our findings demonstrated that the quercetin content in all formulations was less than the amount stated in the labeling information, nevertheless they exerted significant cytotoxic activities in breast cancer cells. We revealed that formulations with high quercetin content induced apoptosis through restoring the tumor suppression activity of p53 and activating downstream caspases, whereas the formulation with low quercetin content potentially induced non-apoptotic cell death. Our work illustrates the involvement of quercetin in apoptosis through the mitochondrial pathway in accordance with the quercetin content detected by the RP-HPLC.
dc.description.sponsorshipIstanbul Medipol University
dc.identifier.citationErim, Ü. C. ve Ayşit, N. (2024). Development and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements. Journal of Research in Pharmacy, 28(5), 1653-1664. http://dx.doi.org/10.29228/jrp.841
dc.identifier.doi10.29228/jrp.841
dc.identifier.endpage1664
dc.identifier.issn2630-6344
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85204444203
dc.identifier.scopusqualityQ3
dc.identifier.startpage1653
dc.identifier.urihttp://dx.doi.org/10.29228/jrp.841
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13002
dc.identifier.volume28
dc.identifier.wosWOS:001315469600029
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorErim, Ümit Can
dc.institutionauthorAyşit, Neşe
dc.institutionauthorid0000-0002-4121-588X
dc.institutionauthorid0000-0002-4243-9092
dc.language.isoen
dc.relation.ispartofJournal of Research in Pharmacy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectApoptosis
dc.subjectBreast Cancer
dc.subjectFlavonoids
dc.subjectMethod Validation
dc.subjectQuercetin
dc.subjectRP-HPLC
dc.subjectSupplement
dc.titleDevelopment and validation of an RP-HPLC method to compare the apoptotic activity of quercetin found in marketed dietary supplements
dc.typeArticle

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