A preliminary proteomic evaluation of smooth muscle cells in thoracic aortic aneurysms

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dc.authorid0000-0002-8814-7351
dc.contributor.authorAçılan Ayhan, Ceyda
dc.contributor.authorBaykal, Betül
dc.contributor.authorSerhatlı, Müge
dc.contributor.authorKaçar, Ömer
dc.contributor.authorAdıgüzel, Zelal
dc.contributor.authorTaş, Serpil
dc.contributor.authorBaysal, Kemal
dc.contributor.authorBaykal, Ahmet Tarık
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:03:40Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:03:40Z
dc.date.issued2014
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.descriptionWOS: 000332944300010
dc.description.abstractAortic aneurysm is characterized as localized degeneration of the aorta leading to advanced weakening and widening of the vessel. While the exact mechanisms have yet to be determined, current studies indicate that the degradation of extracellular matrix (ECM) proteins and apoptosis of vascular smooth muscle cells (SMCs) may result in extendibility, dilation, and rupture of the vessel. Within the aortic wall, SMCs are implicated as key components involved in disease development, as numerous molecular changes have been reported to occur. Most current studies involve either investigation of proteins constituting a group or pathway in SMCs, or analyses of the whole aortic tissue. In order to determine which proteins are important in the development of thoracic aortic aneurysms (TAAs), we performed comparative proteomic analyses using cultured SMCs from TAAs versus controls. Label-free nano LC-MS/MS analysis of cell extracts resulted in the identification of 256 proteins, 26 of which were differentially regulated by >= 1.4-fold. Both previously described and new proteins were identified that were involved in oxidative stress, ECM formation, energy metabolism, or the 14-3-3 pathway. Among these, differential expression of SerpinH1, a protease inhibitor for collagenases, was further verified via immunoblotting. Here we have attempted to shed light on the cellular mechanisms of TAAs.
dc.description.sponsorshipEuropean Union [200647]en_US
dc.description.sponsorshipThis work was supported by the European Union 7th Framework Programme Project entitled Fighting Aneurysmal Disease (FAD), Health-2007-2.4.2-2, Grant Agreement No. 200647, and internal funding through TUBITAK.en_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)en_US
dc.identifier.citationAçılan Ayhan, C., Baykal, B., Serhatlı, M., Kaçar, Ö., Adıgüzel, Z., Taş, S. ... Baykal, A. T. (2014). A preliminary proteomic evaluation of smooth muscle cells in thoracic aortic aneurysms. Turkish Journal of Biology, 38(2), 238-252. https://dx.doi.org/10.3906/biy-1306-24
dc.identifier.doi10.3906/biy-1306-24
dc.identifier.endpage252
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue2
dc.identifier.scopusqualityQ2
dc.identifier.startpage238
dc.identifier.urihttps://dx.doi.org/10.3906/biy-1306-24
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3920
dc.identifier.volume38
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherThe Scientific and Technological Research Council of Turkey
dc.relation.ecinfo:eu-repo/grantAgreement/EC/FP7/200647
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLabel-Free Proteomics
dc.subjectThoracic Aortic Aneurysm
dc.subjectSmooth Muscle Cell
dc.subjectSerpinH1/HSP47
dc.subjectOxidative Stress
dc.subjectProtein Expression
dc.titleA preliminary proteomic evaluation of smooth muscle cells in thoracic aortic aneurysms
dc.typeArticle

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