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dc.contributor.authorAlmosa, Heba
dc.contributor.authorAlqriqri, Mihal
dc.contributor.authorDenetiu, Iuliana
dc.contributor.authorBaghdadi, Mohammed Abbas
dc.contributor.authorAlkhaled, Mohammed
dc.contributor.authorAlhosin, Mahmoud
dc.contributor.authorAldajani, Wejdan A.
dc.contributor.authorZamzami, Mazin
dc.contributor.authorÜçışık, Mehmet Hikmet
dc.date.accessioned2020-08-05T07:03:47Z
dc.date.available2020-08-05T07:03:47Z
dc.date.issued2020en_US
dc.identifier.citationAlmosa, H., Alqriqri, M., Denetiu, I., Baghdadi, M. A., Alkhaled, M., Alhosin, M. ... Üçışık, M. H. (2020). Cytotoxicity of standardized curcuminoids mixture against epithelial ovarian cancer cell line SKOV-3. Scientia Pharmaceutica, 88(1). https://dx.doi.org/10.3390/scipharm88010011en_US
dc.identifier.issn2218-0532
dc.identifier.urihttps://dx.doi.org/10.3390/scipharm88010011
dc.identifier.urihttps://hdl.handle.net/20.500.12511/5666
dc.description.abstractHerbal medicine has been in use for centuries for a wide variety of ailments; however, the efficacy of its therapeutic agents in modern medicine is currently being studied. Curcuminoids are an example of natural agents, widely used due to their potential contribution in the prevention and treatment of cancer. In this study, the three main compounds of curcuminoids-curcumin, desmethoxycurcumin, and bisdesmethoxycurcumin-were determined by reversed-phase high performance liquid chromatography (HPLC) to quantify total content in a mixture. Subsequently, the effect of the three curcuminoids, employed as one sample, was evaluated, to study the proliferation, apoptosis, cell cycle, and migration of the human ovarian cancer cell line SKOV-3. The results reveal that curcuminoids inhibit the proliferation of SKOV-3 cells with concentration- and time-dependent mechanisms. The morphological analysis of the treated SKOV-3 cells showed a typical apoptotic phenotype-cell shrinkage and membrane blebbing in a dose-dependent manner. In addition, flow cytometry demonstrated an increase in apoptosis with an IC50 of 30 mu M curcuminoids. The migration of SKOV-3 cells was also inhibited, reflected by a decrease in wound area. Furthermore, the curcuminoids were found to have no stimulation effect on the expression of cytokines TNF-alpha and IL-10. These results suggest that a curcuminoid mixture can effectively suppress epithelial cancer cell growth in vitro by inducing cellular changes and apoptosis.en_US
dc.description.sponsorshipDeanship of Scientific Research (DSR) at King Abdulaziz University (KAU), Jeddah, SAen_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectCurcuminoidsen_US
dc.subjectOvarian Canceren_US
dc.subjectSKOV-3en_US
dc.subjectFree Radical Scavengersen_US
dc.subjectApoptosisen_US
dc.subjectCell Cycleen_US
dc.subjectCell Migrationen_US
dc.titleCytotoxicity of standardized curcuminoids mixture against epithelial ovarian cancer cell line SKOV-3en_US
dc.typearticleen_US
dc.relation.journalScientia Pharmaceuticaen_US
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümüen_US
dc.authorid0000-0001-9434-3861en_US
dc.identifier.volume88en_US
dc.identifier.issue1en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.3390/scipharm88010011en_US


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