dc.contributor.author | Sibai, Mustafa | |
dc.contributor.author | Altuntaş, Ebru | |
dc.contributor.author | Süzek, Barış Ethem | |
dc.contributor.author | Şahin, Betül | |
dc.contributor.author | Parlayan, Cüneyd | |
dc.contributor.author | Öztürk, Gürkan | |
dc.contributor.author | Baykal, Ahmet Tarık | |
dc.contributor.author | Demircan, Turan | |
dc.date.accessioned | 2019-12-27T06:12:43Z | |
dc.date.available | 2019-12-27T06:12:43Z | |
dc.date.issued | 2020 | en_US |
dc.identifier.citation | Sibai, M., Altuntaş, E., Süzek, B. E., Şahin, B., Parlayan, C., Öztürk, G. ... Demircan, T. (2020). Comparison of protein expression profile of limb regeneration between neotenic and metamorphic axolotl. Biochemical and Biophysical Research Communications, 522(2), 428-434. http://doi.org/10.1016/j.bbrc.2019.11.118 | en_US |
dc.identifier.issn | 0006-291X | |
dc.identifier.issn | 1090-2104 | |
dc.identifier.uri | http://doi.org/10.1016/j.bbrc.2019.11.118 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/4749 | |
dc.description.abstract | The axolotl (Ambystoma mexicanum) salamander, a urodele amphibian, has an exceptional regenerative capacity to fully restore an amputated limb throughout the life-long lasting neoteny. By contrast, when axolotls are experimentally induced to metamorphosis, attenuation of the limb's regenerative competence is noticeable. Here, we sought to discern the proteomic profiles of the early stages of blastema formation of neotenic and metamorphic axolotls after limb amputation by employing LC-MS/MS technology. We quantified a total of 714 proteins and qRT-PCR for selected genes was performed to validate the proteomics results and provide evidence for the putative link between immune system activity and regenerative potential. This study provides new insights for examination of common and distinct molecular mechanisms in regeneration-permissive neotenic and regeneration-deficient metamorphic stages at the proteome level. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | info:eu-repo/semantics/embargoedAccess | en_US |
dc.subject | Proteomics | en_US |
dc.subject | Axolotl | en_US |
dc.subject | Limb Regeneration | en_US |
dc.subject | Metamorphosis | en_US |
dc.subject | Gene Expression Profile | en_US |
dc.title | Comparison of protein expression profile of limb regeneration between neotenic and metamorphic axolotl | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER) | en_US |
dc.department | İstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü | en_US |
dc.department | İstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı | en_US |
dc.authorid | 0000-0002-6183-9489 | en_US |
dc.authorid | 0000-0003-0352-1947 | en_US |
dc.identifier.volume | 522 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.startpage | 428 | en_US |
dc.identifier.endpage | 434 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1016/j.bbrc.2019.11.118 | en_US |
dc.identifier.wosquality | Q2 | en_US |
dc.identifier.scopusquality | Q1 | en_US |