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dc.contributor.authorKurt, Hasan
dc.contributor.authorEyüpoğlu, Alp Ertunga
dc.contributor.authorSütlü, Tolga
dc.contributor.authorBudak, Hikmet
dc.contributor.authorYüce, Meral
dc.date.accessioned2019-12-19T07:57:19Z
dc.date.available2019-12-19T07:57:19Z
dc.date.issued2019en_US
dc.identifier.citationKurt, H., Eyüpoğlu, A. E., Sütlü, T., Budak, H., Yüce, M. (2019). Plasmonic selection of ssDNA aptamers against fibroblast growth factor receptor. ACS Combinatorial Science, 21(8), 578-587. http://dx.doi.org/10.1021/acscombsci.9b00059en_US
dc.identifier.issn2156-8952
dc.identifier.issn2156-8944
dc.identifier.urihttp://dx.doi.org/10.1021/acscombsci.9b00059
dc.identifier.urihttps://hdl.handle.net/20.500.12511/4537
dc.description.abstractIn this work, we describe the selection of ssDNA aptamers targeting fibroblast growth factor receptor binding protein 3 K650E, which has roles in cell division, growth, and differentiation through the kinase cascade. The selection process was based on the label-free, real-time monitoring of binding interactions by surface plasmon resonance, allowing for convenient manipulation of the selection rounds. Next generation sequencing data provided four major motif families from which nine individual sequences were selected based on their abundance levels. Electrophoretic mobility shift assays revealed binding of the selected aptamers to the target protein without significant interference from fibroblast growth factor receptor binding protein 2, indicating the selectivity of the aptamers. The dissociation constant at equilibrium for the best aptamer candidate, SU-3, was found to be (28.2 ± 19.6) × 10–9 M (n = 5) using a single-cycle kinetic analysis method. Advantages of the experimental setup and potential applications of the selected aptamers are discussed.en_US
dc.description.sponsorshipSabanci Universityen_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectAptameren_US
dc.subjectNext Generation Sequencingen_US
dc.subjectSelexen_US
dc.subjectssDNAen_US
dc.subjectSurface Plasmon Resonanceen_US
dc.titlePlasmonic selection of ssDNA aptamers against fibroblast growth factor receptoren_US
dc.typearticleen_US
dc.relation.ispartofACS Combinatorial Scienceen_US
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümüen_US
dc.authorid0000-0002-1677-644Xen_US
dc.identifier.volume21en_US
dc.identifier.issue8en_US
dc.identifier.startpage578en_US
dc.identifier.endpage587en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1021/acscombsci.9b00059en_US
dc.identifier.wosqualityQ2en_US
dc.identifier.scopusqualityQ1en_US


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