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dc.contributor.authorBeker, Mustafa Çağlar
dc.contributor.authorÇağlayan, Berrak
dc.contributor.authorYalçın, Esra
dc.contributor.authorÇağlayan, Ahmet Burak
dc.contributor.authorTürkseven, Şeyma
dc.contributor.authorGürel, Büşra
dc.contributor.authorKeleştemur, Taha
dc.contributor.authorSertel, Elif
dc.contributor.authorŞahin, Zafer
dc.contributor.authorKutlu, Selim
dc.contributor.authorKılıç, Ülkan
dc.contributor.authorBaykal, Ahmet Tarık
dc.contributor.authorKılıç, Ertuğrul
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:50:47Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:50:47Z
dc.date.issued2018en_US
dc.identifier.citationBeker, M. Ç., Çağlayan, B., Yalçın, E., Çağlayan, A. B., Türkseven, Ş., Gürel, B. … Kılıç, E. (2018). Time-of-day dependent neuronal injury after ischemic stroke: Implication of circadian clock transcriptional factor bmal1 and survival kinase akt. Molecular Neurobiology, 55(3), 2565-2576. https://dx.doi.org/10.1007/s12035-017-0524-4en_US
dc.identifier.issn0893-7648
dc.identifier.issn1559-1182
dc.identifier.urihttps://dx.doi.org/10.1007/s12035-017-0524-4
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2076
dc.descriptionWOS: 000426897800060en_US
dc.descriptionPubMed ID: 28421530en_US
dc.description.abstractOccurrence of stroke cases displays a time-of-day variation in human. However, the mechanism linking circadian rhythm to the internal response mechanisms against pathophysiological events after ischemic stroke remained largely unknown. To this end, temporal changes in the susceptibility to ischemia/reperfusion (I/R) injury were investigated in mice in which the ischemic stroke induced at four different Zeitgeber time points with 6-h intervals (ZT0, ZT6, ZT12, and ZT18). Besides infarct volume and brain swelling, neuronal survival, apoptosis, ischemia, and circadian rhythm related proteins were examined using immunohistochemistry, Western blot, planar surface immune assay, and liquid chromatography-mass spectrometry tools. Here, we present evidence that midnight (ZT18; 24:00) I/R injury in mice resulted in significantly improved infarct volume, brain swelling, neurological deficit score, neuronal survival, and decreased apoptotic cell death compared with ischemia induced at other time points, which were associated with increased expressions of circadian proteins Bmal1, PerI, and Clock proteins and survival kinases AKT and Erk-1/2. Moreover, ribosomal protein S6, mTOR, and Bad were also significantly increased, while the levels of PRAS40, negative regulator of AKT and mTOR, and phosphorylated p53 were decreased at this time point compared to ZT0 (06:00). Furthermore, detailed proteomic analysis revealed significantly decreased CSKP, HBB-1/2, and HBA levels, while increased GNAZ, NEGR1, IMPCT, and PDE1B at midnight as compared with early morning. Our results indicate that nighttime I/R injury results in less severe neuronal damage, with increased neuronal survival, increased levels of survival kinases and circadian clock proteins, and also alters the circadian-related proteins.en_US
dc.description.sponsorshipNecmettin Erbakan University [161330001]; Turkish Academy of Sciences (TUBA)en_US
dc.description.sponsorshipThis study was supported by The Turkish Academy of Sciences (TUBA) and Necmettin Erbakan University (Scientific Research Project: 161330001).en_US
dc.language.isoengen_US
dc.publisherHumana Press Incen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectCircadian Rhythmen_US
dc.subjectCerebral Ischemiaen_US
dc.subjectProteomicsen_US
dc.subjectAkt Signalingen_US
dc.subjectPI3Ken_US
dc.subjectBmal1en_US
dc.titleTime-of-day dependent neuronal injury after ischemic stroke: Implication of circadian clock transcriptional factor bmal1 and survival kinase akten_US
dc.typearticleen_US
dc.relation.ispartofMolecular Neurobiologyen_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)en_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalıen_US
dc.authorid0000-0002-9476-8488en_US
dc.authorid0000-0002-5072-132Xen_US
dc.authorid0000-0002-1064-7989en_US
dc.authorid0000-0002-6242-3709en_US
dc.authorid0000-0003-3616-1204en_US
dc.authorid0000-0002-6895-8560en_US
dc.authorid0000-0001-6494-8923en_US
dc.identifier.volume55en_US
dc.identifier.issue3en_US
dc.identifier.startpage2565en_US
dc.identifier.endpage2576en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s12035-017-0524-4en_US
dc.identifier.wosqualityQ1en_US
dc.identifier.scopusqualityQ1en_US


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