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dc.contributor.authorÇakmaklı, Hasan Fatih
dc.contributor.authorGökçe, Hafize
dc.contributor.authorSöylemez, Esma
dc.contributor.authorTopçuoğlu, Pervin
dc.contributor.authorKayaaltı, Zeliha
dc.contributor.authorİleri, Dilber Talia
dc.date.accessioned2023-11-07T09:36:29Z
dc.date.available2023-11-07T09:36:29Z
dc.date.issued2023en_US
dc.identifier.citationÇakmaklı, H. F., Gökçe, H., Söylemez, E., Topçuoğlu, P., Kayaaltı, Z. ve İleri, D. T. (2023). The effect of granulocyte colony stimulating factor on genotoxicity in allogeneic peripheral blood stem cell transplantation donors: A prospective case-control study. Turkish Journal of Pediatrics, 65(5), 809-821. https://dx.doi.org/10.24953/turkjped.2023.469en_US
dc.identifier.issn0041-4301
dc.identifier.issn2791-6421
dc.identifier.urihttps://dx.doi.org/10.24953/turkjped.2023.469
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11717
dc.description.abstractBackground. Every year, thousands of donors are exposed to granulocyte-colony stimulating factor (G-CSF) for stem cell mobilization in hematopoietic stem cell transplantations (HSCT). Previous studies about the genotoxicity of G-CSF were inconclusive. In this study, the genotoxic effects of G-CSF in peripheral blood stem cell (PBSC) donors were evaluated prospectively by using three different validated and reliable methods for the first time in the literature to the best of our knowledge. Methods. Donors of PBSC transplantation (n=36), who received G-CSF were evaluated for genotoxicity by micronucleus test (MNT), nuclear division index (NDI), and comet assay (CA). Genotoxic effects are expected to cause an increase in MNT and CA values and decrease in NDI. Blood samples were collected at three time-points (TP): before starting G-CSF (TP1), after G-CSF for five days (TP2), and one month after the last dose (TP3). Sixteen controls were included for baseline comparison of genotoxicity tests. CD34 cell counts and hemograms were also analyzed. Results. MNT and CA parameters; comet and tail length, tail DNA%, and tail moment, showed no change in time whereas another CA parameter, Olive’s tail moment (OTM) was increased significantly at TP3 compared to both baseline and TP2 (p=0.002 and p=0.017, respectively). Nuclear division index decreased significantly at TP2 (p[removed]en_US
dc.description.sponsorshipAnkara University Scientific Study Funden_US
dc.language.isoengen_US
dc.publisherTurkish National Pediatric Societyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectComet Assayen_US
dc.subjectGenotoxicity Testsen_US
dc.subjectGranulocyte Colony-Stimulating Factoren_US
dc.subjectHematopoietic Stem Cell Donoren_US
dc.subjectHematopoietic Stem Cell Mobilizationen_US
dc.subjectMicronucleus Testsen_US
dc.subjectPeripheral Blood Stem Cell Transplantationen_US
dc.subjectTissue Donoren_US
dc.titleThe effect of granulocyte colony stimulating factor on genotoxicity in allogeneic peripheral blood stem cell transplantation donors: A prospective case-control studyen_US
dc.typearticleen_US
dc.relation.ispartofTurkish Journal of Pediatricsen_US
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Farmasötik Toksikoloji Ana Bilim Dalıen_US
dc.authorid0000-0002-6309-7546en_US
dc.identifier.volume65en_US
dc.identifier.issue5en_US
dc.identifier.startpage809en_US
dc.identifier.endpage821en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.24953/turkjped.2023.469en_US
dc.institutionauthorSöylemez, Esma
dc.identifier.wosqualityQ4en_US
dc.identifier.wos001089229600010en_US
dc.identifier.scopus2-s2.0-85174750048en_US
dc.identifier.pmid37853972en_US
dc.identifier.scopusqualityQ3en_US


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