dc.contributor.author | Gençoğlu Katmerlikaya, Tuğba | |
dc.contributor.author | Dağ, Aydan | |
dc.contributor.author | Omurtag Özgen, Pınar Sinem | |
dc.contributor.author | Çetin Ersen, Büşra | |
dc.date.accessioned | 2023-02-22T07:46:18Z | |
dc.date.available | 2023-02-22T07:46:18Z | |
dc.date.issued | 2022 | en_US |
dc.identifier.citation | Gençoğlu Katmerlikaya, T., Dağ, A., Omurtag Özgen, P. S. ve Çetin Ersen, B. (2022). Dual-drug conjugated glyco-nanoassemblies for tumor-triggered targeting and synergistic cancer therapy. ACS Applied Bio Materials, 5(11), 5356-5364. https://doi.org/10.1021/acsabm.2c00749 | en_US |
dc.identifier.issn | 2576-6422 | |
dc.identifier.uri | https://doi.org/10.1021/acsabm.2c00749 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/10499 | |
dc.description.abstract | Drug-conjugated nanoassemblies potentiate the efficiency of anticancer drugs through the advantages of high drug-loading capacity and passive/active targeting ability in cancer therapy. This study describes the synthesis of gemcitabine (Gem) and cisplatin (cisPt) dual-drug-functionalized glyco-nanoassemblies (GNs) for anticancer drug delivery systems. It also investigates the pH-triggered drug delivery of the conventional anticancer drug cisPt. A Gem-functionalized well-defined glycoblock copolymer backbone (P(iprFruMA-b-MAc)-Gem), which consists of fructose and methacrylic acid segments, was synthesized via a reversible addition-fragmentation chain transfer (RAFT) polymerization method. Following the hydrolysis of the protecting groups on the backbone copolymer, cisPt functionalization of P(FruMA-b-MAc)-Gem in aqueous media was carried out during the transformation of glycoblock polymers into self-assembled spherical glyco-nanoassemblies (GN3). Monodrug-functionalized glyco-nanoassemblies were also prepared either with Gem (GN1) or cisPt (GN2) to compare the synergetic effect of dual-drug conjugated glyco-nanoassemblies (GN3). The sizes of glyco-nanoassemblies GN1, GN2, and GN3 were found as 5.76 +/- 0.64, 59.80 +/- 0.13, and 53.80 +/- 3.90 nm and dispersity (D) values as 0.476, 0.292, and 0.311 by dynamic light scattering (DLS) measurement, respectively. The in vitro studies revealed that the drug-free glyco-nanoassemblies are biocompatible at concentrations higher than 296 mu g/mL. The drug-conjugated glyco-nanoassemblies (GN1 and GN2) exhibited in vitro cytotoxicity against human breast cancer cell lines of MDA-MB-231 comparable to free Gem and cisPt, illustrating an efficient drug release into the tumor environment. Additionally, GNs exhibited higher selectivity and preferential cellular internalization in MDA-MB-231 when compared to healthy cell lines of CCD-1079Sk. These dual-drug conjugated GNs can effectively enhance the killing of cancer cells and increase synergistic chemotherapy. | en_US |
dc.description.sponsorship | Bezmialem Vakif University ; Scientific and Technological Research Council of Turkiye | en_US |
dc.language.iso | eng | en_US |
dc.publisher | American Chemical Society | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Glyco-Nanoassemblies | en_US |
dc.subject | Gemcitabinecis | en_US |
dc.subject | CisPt | en_US |
dc.subject | Cellular Uptake | en_US |
dc.subject | Synergistic Chemotherapy | en_US |
dc.title | Dual-drug conjugated glyco-nanoassemblies for tumor-triggered targeting and synergistic cancer therapy | en_US |
dc.type | article | en_US |
dc.relation.ispartof | ACS Applied Bio Materials | en_US |
dc.department | İstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümü, Analitik Kimya Ana Bilim Dalı | en_US |
dc.authorid | 0000-0003-2493-9664 | en_US |
dc.identifier.volume | 5 | en_US |
dc.identifier.issue | 11 | en_US |
dc.identifier.startpage | 5356 | en_US |
dc.identifier.endpage | 5364 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1021/acsabm.2c00749 | en_US |
dc.institutionauthor | Omurtag Özgen, Pınar Sinem | |
dc.identifier.wos | 000884435000001 | en_US |
dc.identifier.scopus | 2-s2.0-85141967192 | en_US |
dc.identifier.pmid | 36346990 | en_US |
dc.identifier.scopusquality | Q1 | en_US |