Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels

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dc.authorid0000-0003-2033-860X
dc.authorid0000-0002-6988-3636
dc.authorid0000-0001-9037-5217
dc.authorid0000-0003-0352-1947
dc.authorid0000-0002-9373-0808
dc.contributor.authorSevimli, Gülşah
dc.contributor.authorSmith, Matthew J.
dc.contributor.authorAkgül Çağlar, Tuba
dc.contributor.authorBilir, Şükriye
dc.contributor.authorSeçilmiş, Melike
dc.contributor.authorAltun, Hamza Y.
dc.contributor.authorYiğit, Esra N.
dc.contributor.authorYang, Fan
dc.contributor.authorKeeley, Thomas P.
dc.contributor.authorMalli, Roland
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorMann, Giovanni E.
dc.contributor.authorEroğlu, Emrah
dc.date.accessioned2022-05-20T11:36:24Z
dc.date.available2022-05-20T11:36:24Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.description.abstractIron is an essential metal for cellular metabolism and signaling, but it has adverse effects in excess. The physiological consequences of iron deficiency are well established, yet the relationship between iron supplementation and pericellular oxygen levels in cultured cells and their downstream effects on metalloproteins has been less explored. This study exploits the metalloprotein geNOps in cultured HEK293T epithelial and EA.hy926 endothelial cells to test the iron-dependency in cells adapted to standard room air (18 kPa O2) or physiological normoxia (5 kPa O2). We show that cells in culture require iron supplementation to activate the metalloprotein geNOps and demonstrate for the first time that cells adapted to physiological normoxia require significantly lower iron compared to cells adapted to hyperoxia. This study establishes an essential role for recapitulating oxygen levels in vivo and uncovers a previously unrecognized requirement for ferrous iron supplementation under standard cell culture conditions to achieve geNOps functionality.
dc.description.sponsorshipIntegration Projects of Sabanci University ; Heart Research U.K. ; British Heart Foundation ; European Cooperation in Science and Technology (COST) ; King's Together Strategic Awarden_US
dc.identifier.citationSevimli, G., Smith, M. J., Akgül Çağlar, T., Bilir, Ş., Seçilmiş, M., Altun, H. Y. ... Eroğlu, E. (2022). Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels. Redox Biology, 53. https://doi.org/10.1016/j.redox.2022.102319
dc.identifier.doi10.1016/j.redox.2022.102319
dc.identifier.issn2213-2317
dc.identifier.pmid35525027
dc.identifier.scopus2-s2.0-85129621442
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.redox.2022.102319
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9456
dc.identifier.volume53
dc.identifier.wos000805648100002en_US
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAkgül Çağlar, Tuba
dc.institutionauthorBilir, Şükriye
dc.institutionauthorYiğit, Esra N.
dc.institutionauthorÖztürk, Gürkan
dc.institutionauthorEroğlu, Emrah
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofRedox Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/118C242
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCell Culture
dc.subjectCulture Media
dc.subjectFerric Iron
dc.subjectFerrous Iron
dc.subjectGeNOps
dc.subjectHydrogen Peroxide
dc.subjectHyperoxia
dc.subjectNO Bioavailability
dc.subjectNormoxia
dc.subjectPericellular Oxygen
dc.titleNitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels
dc.typeArticle

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