Are chondrocytes damaged when rheumatologic inflammation is suppressed?

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dc.authorid0000-0001-9052-4411
dc.authorid0000-0002-7794-9308
dc.contributor.authorYıldırım Güzelant, Aliye
dc.contributor.authorİşyar, Mehmet
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorÇakmak, Selami
dc.contributor.authorMahiroğulları, Mahir
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:51:51Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:51:51Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Ortopedi ve Travmatoloji Ana Bilim Dalı
dc.descriptionWOS: 000395010200003
dc.descriptionPubMed ID: 27079996
dc.description.abstractAim: The use of biological agents (BAs) for treating diseases such as rheumatoid arthritis (RA), spondyloarthropathy, and systemic lupus erythematosus to reduce inflammation has been fruitful. Especially as part of the increasing number of studies on the intra-articular application of BAs, the effects of BAs on cartilage have been widely investigated. In the present study, the effects of rituximab, abatacept, and adalimumab, all approved antirheumatic agents, on human primary chondrocytes were investigated comparatively and on the molecular level through viability, proliferation, and toxicity analyses. Materials and methods: Osteochondral tissues from the distal femur and proximal tibia were resected during total knee arthroplasty from patients (n=3) with confirmed gonarthrosis in whom all medical or conservative treatments had failed. Standard human primary chondrocyte cell culturing was carried out. Immunophenotyping was performed on the cells that adhered to the flask, and their chondrotoxicity was observed using a flow cytometry device. Images of the cells showing chondrotoxicity were analyzed using invert and environmental scanning microscopes, and microimages were obtained. The MTT-enzyme linked immunosorbent assay was performed to observe the toxic effects of BAs on the proliferation of chondrocytes at 24 and 48h. The results were analyzed using the number of cells and proliferation; statistical comparisons among the groups were carried out using one-way ANOVA. The alpha significance level was set at <0.01. Results: These pharmaceutical agents were chondrotoxic, especially on viability and proliferation (p=0.0000). Conclusion: BAs are generally used during active inflammation, and following the management of inflammation, their dosage should be determined taking into consideration their cellular-level toxic effects on chondrocytes.
dc.identifier.citationYıldırım Güzelant, A., İşyar, M., Yılmaz, İ., Yaşar Şirin, D., Çakmak, S. ve Mahiroğulları, M. (2017). Are chondrocytes damaged when rheumatologic inflammation is suppressed? Drug and Chemical Toxicology, 40(1), 13-23. https://dx.doi.org/10.3109/01480545.2016.1166249
dc.identifier.doi10.3109/01480545.2016.1166249
dc.identifier.endpage23
dc.identifier.issn0148-0545
dc.identifier.issn1525-6014
dc.identifier.issue1
dc.identifier.scopusqualityQ2
dc.identifier.startpage13
dc.identifier.urihttps://dx.doi.org/10.3109/01480545.2016.1166249
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2297
dc.identifier.volume40
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofDrug and Chemical Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAbatacept
dc.subjectAdalimumab
dc.subjectCell Culture
dc.subjectChondrotoxicity
dc.subjectRituximab
dc.titleAre chondrocytes damaged when rheumatologic inflammation is suppressed?
dc.typeArticle

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