Bioconjugated beta-cyclodextrin-perfluorohexane nanocone clusters as functional nanoparticles for nanoparticle-mediated histotripsy

Basit Öğe Kaydı
dc.authorid0000-0002-4634-3864
dc.authorid0000-0003-2776-5807
dc.contributor.authorToydemir, Cemran
dc.contributor.authorHall, Sarah
dc.contributor.authorDemirel, Erhan
dc.contributor.authorElmacı, Dilşah Nur
dc.contributor.authorGöl, Deniz
dc.contributor.authorVlaisavljevich, Eli
dc.contributor.authorYüksel Durmaz, Yasemin
dc.date.accessioned2023-09-20T08:19:03Z
dc.date.available2023-09-20T08:19:03Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractNanocone clusters (NCCs) are new-generation agents of nanoparticle-mediated histotripsy (NMH) recently developed to address the limitations of previously designed nanodroplets (NDs). NCCs can be obtained by simply mixing FDA-approved cyclodextrins (CD) and suitable perfluorocarbons (PFCs), which result in smaller size aggregates, detectable PFC amount, and more stable long-term storage since the obtained powder can be stored and redispersed as needed. Previous experimental and computational studies showed that NCCs consist of an organization of inclusion complexes of CD and PFC around free PFC droplets, and their aggregate behavior depends on the localization of PFC in the cavity and the water solubility of CD derivatives. It has been shown that beta-cyclodextrin (beta CD) and perfluorohexane (PFH) are ideal candidates for NCCs that can be isolated as a powder with high PFC content among various CD and PFC derivatives. This study focuses on the further development of the selected NCC composition to enhance the potential of NMH therapy while also enabling more detailed future experiments in vitro and in vivo. It is aimed to show the bioconjugation potential of NCCs through the example of the most commonly used functionalization methods such as targeting, PEGylation, and fluorescent labeling. For this purpose, beta CD as a building block was monofunctionalized with groups such as azide, alkyne, and amine groups that allow for effective coupling reactions such as the "click" reaction and N-(3-dimethylaminopropyl)-N '-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) coupling. These monofunctional beta CDs were used as building blocks of NCCs in the presence of PFH to obtain functional NCCs as precursors of bioconjugation. EPPT1 as a synthetic peptide specific to uMUC1 and folic acid (FA) as the most commonly used targeting agent along with PEGylation were successfully shown as bioconjugation examples. Lastly, fluorescently labeled NCCs were obtained via fluorescein isothiocyanate (FITC) and alkyne functional NCC reaction through propargyl amine and isothiocyanate group reaction. The obtained bioconjugates were tested in vitro to validate the conjugation, and the ability to lower the histotripsy cavitation threshold, which is necessary for NMH, was demonstrated for all bioconjugates. Overall, the results showed that all obtained bioconjugates successfully lowered the cavitation threshold pressure while also fulfilling the desired bioconjugation metrics to serve as improved tools to enhance NMH as a targeted noninvasive ablation method.
dc.description.sponsorshipUnited States Department of Health & Human Services National Institutes of Health (NIH) - USA NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB)en_US
dc.identifier.citationToydemir, C., Hall, S., Demirel, E., Elmacı, D. N., Göl, D., Vlaisavljevich, E. ... Yüksel Durmaz, Y. (2022). Bioconjugated beta-cyclodextrin-perfluorohexane nanocone clusters as functional nanoparticles for nanoparticle-mediated histotripsy. Biomacromolecules, 23(12), 5297-5311. https://doi.org/10.1021/acs.biomac.2c01110
dc.identifier.doi10.1021/acs.biomac.2c01110
dc.identifier.endpage5311
dc.identifier.issn1525-7797
dc.identifier.issn1526-4602
dc.identifier.issue12
dc.identifier.pmid36418020
dc.identifier.scopus2-s2.0-85143080970
dc.identifier.scopusqualityQ1
dc.identifier.startpage5297
dc.identifier.urihttps://doi.org/10.1021/acs.biomac.2c01110
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11467
dc.identifier.volume23
dc.identifier.wos000892861000001en_US
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorToydemir, Cemran
dc.institutionauthorDemirel, Erhan
dc.institutionauthorElmacı, Dilşah Nur
dc.institutionauthorGöl, Deniz
dc.institutionauthorYüksel Durmaz, Yasemin
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.ispartofBiomacromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/118Z324
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectNanoparticle-Mediated Histotripsy
dc.subjectNanocone Clusters
dc.subjectBioconjugated beta-Cyclodextrin-Perfluorohexane
dc.titleBioconjugated beta-cyclodextrin-perfluorohexane nanocone clusters as functional nanoparticles for nanoparticle-mediated histotripsy
dc.title.alternativeBioconjugated ?-cyclodextrin-perfluorohexane nanocone clusters as functional nanoparticles for nanoparticle-mediated histotripsy
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
Toydemir-Cemran-2022.pdf
Boyut:
3.59 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Makale Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu