Iopromide- and gadopentetic acid-derived preparates used in MR arthrography may be harmful to chondrocytes

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dc.authorid0000-0003-2003-6337
dc.authorid0000-0002-8084-7855
dc.contributor.authorÖznam, Kadir
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorKaya, Yasin Emre
dc.contributor.authorİşyar, Mehmet
dc.contributor.authorGümüştaş, Seyit Ali
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorAkkaya, Semih
dc.contributor.authorKayhan, Arda
dc.contributor.authorMahiroğulları, Mahir
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:01:25Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:01:25Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Ortopedi ve Travmatoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Farmakoloji Ana Bilim Dalı
dc.descriptionWOS: 000405006500003
dc.descriptionPubMed ID: 28651625
dc.description.abstractBackground: Magnetic resonance arthrography, a procedure through which contrast agents containing gadolinium and/or iopromide are administered intra-articularly, has become a useful tool in musculoskeletal diagnosis. Nevertheless, despite being considered safe for systemic use, certain tissue toxicities have been identified for both drugs. In this study, the effects of short-term exposure of human primary chondrocyte cell cultures to gadolinium and/or iopromide contrast agents were examined by assaying for stage-specific embryonic antigen-1 (SSEA-1) protein expression (a chondrogenic differentiation marker), cell viability, toxicity, and proliferation. Methods: Human articular chondrocytes were grown in monolayer culture and were exposed to iopromide and/or gadolinium diethylenetriamine-pentaacetate (Gd-DPT) for 2 and 6 h. Cell cultures with no drug exposure were used as the control group. Cell differentiation status was assessed according to SSEA-1 protein expression. Contrast agent effects on cell viability and proliferation were analyzed using MTT analysis. Further, changes in cell morphology in relation to the control group were evaluated using inverted light microscopy, environmental scanning electron microscopy (ESEM), and 3-tesla magnetic resonance imaging. The obtained data were statistically compared. Results: When compared with the control group, both SSEA-1 protein expression and cell proliferation were lowest in the Gd-DPT group (P = 0.000). There was a statistically significant correlation between SSEA-1 expression and MTT results (rho = 0.351; P = 0.003). Conclusions: Nevertheless, the data obtained from in vitro experiments may not directly correspond to clinical applications. However, the mere fact that a drug used solely for diagnostic purposes may repress chondrocyte cell proliferation should be carefully considered by clinicians.
dc.identifier.citationÖznam, K., Şirin, D. Y., Yılmaz, İ., Kaya, Y. E., İşyar, M., Gümüştaş, S. A. ... Mahiroğulları, M. (2017). Iopromide- and gadopentetic acid-derived preparates used in MR arthrography may be harmful to chondrocytes. Journal of Orthopaedic Surgery and Research, 12. https://dx.doi.org/10.1186/s13018-017-0600-5
dc.identifier.doi10.1186/s13018-017-0600-5
dc.identifier.issn1749-799X
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://dx.doi.org/10.1186/s13018-017-0600-5
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3250
dc.identifier.volume12
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBMC
dc.relation.ispartofJournal of Orthopaedic Surgery and Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsCC0 1.0 Universal*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/publicdomain/zero/1.0/*
dc.subjectMR-Arthrography
dc.subjectGadopentetic Acid
dc.subjectIopromide
dc.subjectChondrotoxicity
dc.subjectPrimary Cell Culture
dc.subjectStage-Specific
dc.subjectEmbryonic Antigen-1
dc.titleIopromide- and gadopentetic acid-derived preparates used in MR arthrography may be harmful to chondrocytes
dc.typeArticle

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