Endotoxin of porphyromonas gingivalis amplifies the inflammatory response in hyperglycemia-induced zebrafish through a mechanism involving chitinase-like protein YKL-40 analogs

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dc.authorid0000-0002-6274-801X
dc.contributor.authorGündüz, Gizem
dc.contributor.authorBeler, Merih
dc.contributor.authorÜnal, İsmail
dc.contributor.authorCansız, Derya
dc.contributor.authorEmekli Alturfan, Ebru
dc.contributor.authorKöse, Kemal Naci
dc.date.accessioned2024-01-18T10:27:49Z
dc.date.available2024-01-18T10:27:49Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractPorphyromonas gingivalis (P. gingivalis), a key pathogen in periodontal diseases, is also associated with hyperglycemia-associated systemic diseases, including diabetes mellitus (DM). Gingipains are the most important endotoxins of P. gingivalis, and in vivo studies using gingipains are scarce. Zebrafish (Danio rerio) is a vertebrate with high physiological and genetic homology with humans that has multiple co-orthologs for human genes, including inflammation-related proteins. The aim of our study was to determine the effects of gingipain in a hyperglycemia-induced zebrafish model by evaluating inflammation, oxidant-antioxidant status, and the cholinergic system. Adult zebrafish were grouped into the control group (C), hyperglycemia-induced group subjected to 15 days of overfeeding (OF), gingipain-injected group (GP), and gingipain-injected hyperglycemic group (OF + GP). At the end of 15 days, an oral glucose tolerance test (OGTT) was performed, and fasting blood glucose (FBG) levels were measured. Lipid peroxidation (LPO), nitric oxide (NO), glutathione (GSH), glutathione S-transferase, catalase, acetylcholinesterase (AChE), alkaline phosphatase (ALP), and sialic acid (SA) levels were determined spectrophotometrically in the hepatopancreas. The expression levels of tnf-alpha, il-1 beta, ins, crp, and the acute phase protein YKL-40 analogs chia.5 and chia.6 were evaluated by RT-PCR. After two weeks of overfeeding, significantly increased weight gain, FBG, and OGTT confirmed that the zebrafish were hyperglycemic. Increased oxidative stress, inflammation, and AChE and ALP activities were observed in both the overfeeding and GP groups. Amplification of inflammation and oxidative stress was evident in the OF + GP group through increased expression of crp, il-1 beta, chia.5, and chia.6 and increased LPO and NO levels. Our results support the role of gingipains in the increased inflammatory response in hyperglycemia-associated diseases.
dc.description.sponsorshipTDK-2021-10420en_US
dc.identifier.citationGündüz, G., Beler, M., Ünal, İ., Cansız, D., Emekli Alturfan, E. ve Köse, K. N. (2023). Endotoxin of porphyromonas gingivalis amplifies the inflammatory response in hyperglycemia-induced zebrafish through a mechanism involving chitinase-like protein YKL-40 analogs. Toxicological Research, 39(4), 625-636. https://dx.doi.org/10.1007/s43188-023-00190-4
dc.identifier.doi10.1007/s43188-023-00190-4
dc.identifier.endpage636
dc.identifier.issn1976-8257
dc.identifier.issn2234-2753
dc.identifier.issue4
dc.identifier.pmid37779592
dc.identifier.scopus2-s2.0-85160619608
dc.identifier.scopusqualityQ3
dc.identifier.startpage625
dc.identifier.urihttps://dx.doi.org/10.1007/s43188-023-00190-4
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12169
dc.identifier.volume39
dc.identifier.wos000999175100001en_US
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCansız, Derya
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofToxicological Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectGingipain
dc.subjectHyperglycemia
dc.subjectInflammation
dc.subjectRT?PCR
dc.subjectZebrafish
dc.titleEndotoxin of porphyromonas gingivalis amplifies the inflammatory response in hyperglycemia-induced zebrafish through a mechanism involving chitinase-like protein YKL-40 analogs
dc.typeArticle

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