Detection of O25b-ST131 clone, CTX-M-1 and CTX-M-15 genes via real-time PCR in Escherichia coli strains in patients with UTIs obtained from a university hospital in Istanbul

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dc.authorid0000-0003-3952-1914
dc.contributor.authorDemirci, Mehmet
dc.contributor.authorÜnlü, Özge
dc.contributor.authorİstanbullu Tosun, Ayşe
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:36:46Z
dc.date.available10.07.201910:49:14
dc.date.available2019-07-10T19:36:46Z
dc.date.issued2019
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractBackground: Escherichia coli sequence type 131 is an important multidrug resistant clone responsible from more than half of ESBL-producing E.coli isolates. Aim of this study was to investigate the presence of O25b-ST131 clone, CTX-M-15 and CTX-M-1 genes in the E. coli strains isolated from both hospital and community acquired UTIs by real-time PCR and to reveal molecular epidemiological data. Methods: Non-duplicate E. coli (n = 101) strains isolated from UTI patients were included. Bacterial identifications were performed with VITEK Compact. Antimicrobial susceptibility tests, phenotypic ESBL and E-tests were performed conventionally. Real-time PCR was utilized to detect presence of O25b-ST131 clone, blaCTX-M-15 and blaCTX-M-1. Results: O25b-ST131 clone, CTX-M-1 and CTX-M-15 were detected in 22%, 73%, 37% in UTIs, respectively. Presence of O25b-ST131 clones and CTX-M-1 genes among E. coli strains isolated from inpatients were found statistically higher than outpatients. The most effective choice was found to be fosfomycin and nitrofurantoin in outpatients and inpatients, respectively. The MIC 90 values of Amikacin, Cefotaxime, Cefepime and Ciprofloxacin were higher in inpatients than in oupatients, whereas Cefotaxime and Ciprofloxacin MIC 50 values were found to be higher in inpatients than in outpatients. The highest increase of MIC 90 values was observed in O25b-ST131, CTX-M-1 and CTX-M-15 coexistence. Conclusion: The presence of O25b-ST131 clone, CTX-M-1 and CTX-M-15 genes in E. coli strains in patients with UTI has been revealed. In the presence of the O25b-ST131 clone, a significant increase was observed in the ciprofloxacin MIC values indicating the importance of monitorization of the clone using molecular epidemiology.
dc.identifier.citationDemirci, M., Ünlü, Ö. ve İstanbullu Tosun, A. (2019). Detection of O25b-ST131 clone, CTX-M-1 and CTX-M-15 genes via real-time PCR in Escherichia coli strains in patients with UTIs obtained from a university hospital in Istanbul. Journal of Infection and Public Health, 12(5), 640-644. https://dx.doi.org/10.1016/j.jiph.2019.02.017
dc.identifier.doi10.1016/j.jiph.2019.02.017
dc.identifier.endpage644
dc.identifier.issn1876-0341
dc.identifier.issn1876-035X
dc.identifier.issue5
dc.identifier.scopusqualityQ2
dc.identifier.startpage640
dc.identifier.urihttps://hdl.handle.net/20.500.12511/1252
dc.identifier.urihttps://dx.doi.org/10.1016/j.jiph.2019.02.017
dc.identifier.volume12
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Ltd
dc.relation.ispartofJournal of Infection and Public Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCTX-M-15
dc.subjectEscherichia Coli
dc.subjectO25b-ST131
dc.subjectReal-Time PCR
dc.subjectUTI
dc.titleDetection of O25b-ST131 clone, CTX-M-1 and CTX-M-15 genes via real-time PCR in Escherichia coli strains in patients with UTIs obtained from a university hospital in Istanbul
dc.typeArticle

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