S-layer fusion protein as a tool functionalizing emulsomes and CurcuEmulsomes for antibody binding and targeting

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dc.authorid0000-0001-9434-3861
dc.contributor.authorÜçışık, Mehmet
dc.contributor.authorKüpçü, Seda
dc.contributor.authorBreitwieser, Andreas
dc.contributor.authorGelbmann, Nicola
dc.contributor.authorSchuster, Bernhard
dc.contributor.authorSleytr, Uwe
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:51:30Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:51:30Z
dc.date.issued2015
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.descriptionWOS: 000353930000019
dc.descriptionPubMed ID: 25734967
dc.description.abstractSelective targeting of tumor cells by nanoparticle-based drug delivery systems is highly desirable because it maximizes the drug concentration at the desired target while simultaneously protecting the surrounding healthy tissues. Here, we show a design for smart nanocarriers based on a biomimetic approach that utilizes the building principle of virus envelope structures. Emulsomes and CurcuEmulsomes comprising a tripalmitin solid core surrounded by phospholipid layers are modified by S-layer proteins that self-assemble into a two-dimensional array to form a surface layer. One significant advantage of this nanoformulation is that it increases the solubility of the lipophilic anti-cancer agent curcumin in the CurcuEmulsomes by a factor of 2700. In order to make the emulsomes specific for IgG, the S-layer protein is fused with two protein G domains. This S-layer fusion protein preserves its recrystallization characteristics, forming an ordered surface layer (square lattice with 13 nm unit-by-unit distance). The GG domains are presented in a predicted orientation and exhibit a selective binding affinity for IgG.
dc.description.sponsorshipUS Air Force Office of Scientific Research (AFOSR) [FA9550-09-0342, FA9550-10-1-0223]; Austrian Science Fund (FWF) [P-20256-B11]en_US
dc.description.sponsorshipThe work was supported by US Air Force Office of Scientific Research (AFOSR), Agreement Award Nr.: FA9550-09-0342 and Agreement Award Nr.: FA9550-10-1-0223, and the Austrian Science Fund (FWF), project P-20256-B11. The authors thank Marcin Laskiewicz and Jaqueline Friedmann for their assistance in AFM experiments. The authors also thank to Dr. Monika Debreczeny for the confocal laser scanning microscopy analysis carried out at the Imaging Center of the Vienna Institute of BioTechnology (VIBT).en_US
dc.identifier.citationÜçışık, M. H., Küpçü, S., Breitwieser, A., Gelbmann, N., Schuster, B. ve Sleytr, U. B. (2015). S-layer fusion protein as a tool functionalizing emulsomes and CurcuEmulsomes for antibody binding and targeting. Colloids and Surfaces B: Biointerfaces, 128, 132-139. https://dx.doi.org/10.1016/j.colsurfb.2015.01.055
dc.identifier.doi10.1016/j.colsurfb.2015.01.055
dc.identifier.endpage139
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.scopusqualityQ1
dc.identifier.startpage132
dc.identifier.urihttps://dx.doi.org/10.1016/j.colsurfb.2015.01.055
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2229
dc.identifier.volume128
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofColloids and Surfaces B: Biointerfacesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectEmulsomes
dc.subjectCurcumin
dc.subjectS-layer (fusion) Proteins
dc.subjectImmunoglobulin G (IgG) Targeting
dc.subjectActive Drug Delivery
dc.titleS-layer fusion protein as a tool functionalizing emulsomes and CurcuEmulsomes for antibody binding and targeting
dc.typeArticle

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