Microfluidics and nanoparticles based amperometric biosensor for the detection of cyanobacteria (Planktothrix agardhii NIVA-CYA 116) DNA

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dc.authorid0000-0002-0060-2859
dc.contributor.authorÖlçer, Zehra
dc.contributor.authorEsen, Elif
dc.contributor.authorErsoy, Aylin
dc.contributor.authorBudak, Sinan
dc.contributor.authorSever Kaya, Dilek
dc.contributor.authorYağmur Gök, Mehmet
dc.contributor.authorBarut, Serkan
dc.contributor.authorÜstek, Duran
dc.contributor.authorUluda?, Yıldız
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:35:40Z
dc.date.available10.07.201910:49:14
dc.date.available2019-07-10T19:35:40Z
dc.date.issued2015
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Genetik Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.description.abstractSome of the cyanobacteria produce protease inhibitor oligopeptides such as cyanopeptolins and cause drinking water contamination; hence, their detection has great importance to monitor the well-being of water sources that is used for human consumption. In the current study, a fast and sensitive nucleic acid biosensor assay has been described where cyanopeptolin coding region of one of the cyanobacteria (Planktothrix agardhii NIVA-CYA 116) genome has been used as target for monitoring of the fresh water resources. A biochip that has two sets of Au electrode arrays, each consist of shared reference/counter electrodes and 3 working electrodes has been used for the assay. The biochip has been integrated to a microfluidics system and all steps of the assay have been performed during the reagent flow to achieve fast and sensitive DNA detection. On-line hybridization of the target on to the capture probe immobilized surface resulted in a very short assay duration with respect to the conventional static assays. The binding of the avidin and enzyme modified Au nanoparticles to the biotinylated detection probe and the subsequent injection of the substrate enabled a real-time amperometric measurement with a detection limit of 6×10-12M target DNA (calibration curve r2=0.98). The developed assay enables fast and sensitive detection of cyanopeptolin producing cyanobacteria from freshwater samples and hence shows a promising technology for toxic microorganism detection from environmental samples.
dc.description.sponsorshipTürkiye Cumhuriyeti Kalkınma Bakanlığıen_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu (TUBITAK)en_US
dc.identifier.citationÖlçer, Z., Esen, E., Ersoy, A., Budak, S., Sever Kaya, D., Yağmur Gök, M. ... Uluda?, Y. (2015). Microfluidics and nanoparticles based amperometric biosensor for the detection of cyanobacteria (Planktothrix agardhii NIVA-CYA 116) DNA. Biosensors and Bioelectronics, 70, 426-432. https://dx.doi.org/10.1016/j.bios.2015.03.052
dc.identifier.doi10.1016/j.bios.2015.03.052
dc.identifier.endpage432
dc.identifier.issn0956-5663
dc.identifier.scopusqualityQ1
dc.identifier.startpage426
dc.identifier.urihttps://hdl.handle.net/20.500.12511/897
dc.identifier.urihttps://dx.doi.org/10.1016/j.bios.2015.03.052
dc.identifier.volume70
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofBiosensors and Bioelectronicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/S569000
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAmperometry
dc.subjectCyanobacteria
dc.subjectCyanopeptolin
dc.subjectElectrochemical Sensing
dc.subjectMicrofluidics
dc.subjectReal-Time Electrochemical Profiling
dc.titleMicrofluidics and nanoparticles based amperometric biosensor for the detection of cyanobacteria (Planktothrix agardhii NIVA-CYA 116) DNA
dc.typeArticle

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