Modulation of excitability of stellate neurons in the ventral cochlear nucleus of mice by atp-sensitive potassium channels

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dc.authorid0000-0003-0352-1947
dc.contributor.authorBal, Ramazan
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorÖnalan Etem, Ebru
dc.contributor.authorHim, Aydın
dc.contributor.authorCengiz, Nurettin
dc.contributor.authorKulo?lu, Tuncay
dc.contributor.authorTuzcu, Mehmet
dc.contributor.authorYıldırım, Caner
dc.contributor.authorTektemur, Ahmet
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:50:54Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:50:54Z
dc.date.issued2018
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.descriptionWOS: 000426553200013
dc.descriptionPubMed ID: 29379989
dc.description.abstractMajor voltage-activated ionic channels of stellate cells in the ventral part of cochlear nucleus (CN) were largely characterized previously. However, it is not known if these cells are equipped with other ion channels apart from the voltage-sensitive ones. In the current study, it was aimed to study subunit composition and function of ATP-sensitive potassium channels (K-ATP) in stellate cells of the ventral cochlear nucleus. Subunits of K-ATP channels, Kir6.1, Kir6.2, SUR1, and SUR2, were expressed at the mRNA level and at the protein level in the mouse VCN tissue. The specific and clearly visible bands for all subunits but that for Kir6.1 were seen in Western blot. Using immunohistochemical staining technique, stellate cells were strongly labeled with SUR1 and Kir6.2 antibodies and moderately labeled with SUR2 antibody, whereas the labeling signals for Kir6.1 were too weak. In patch clamp recordings, K-ATP agonists including cromakalim (50 A mu M), diazoxide (0.2 mM), 3-Amino-1,2,4-triazole (ATZ) (1 mM), 2,2-Dithiobis (5-nitro pyridine) (DTNP) (330 A mu M), 6-Chloro-3-isopropylamino- 4H-thieno[3,2-e]-1,2,4-thiadiazine 1,1-dioxide (NNC 55-0118) (1 A mu M), 6-chloro-3-(methylcyclopropyl)amino-4H-thieno[3,2-e]-1,2,4-thiadiazine 1,1-dioxide (NN414) (1 A mu M), and H2O2 (0.88 mM) induced marked responses in stellate cells, characterized by membrane hyperpolarization which were blocked by K-ATP antagonists. Blockers of K-ATP channels, glibenclamide (0.2 mM), tolbutamide (0.1 mM) as well as 5-hydroxydecanoic acid (1 mM), and catalase (500 IU/ml) caused depolarization of stellate cells, increasing spontaneous action potential firing. In conclusion, K-ATP channels seemed to be composed dominantly of Kir 6.2 subunit and SUR1 and SUR2 and activation or inhibition of K-ATP channels regulates firing properties of stellate cells by means of influencing resting membrane potential and input resistance.
dc.description.sponsorshipTUBI-TAK, Turkey [109S516, 110S397]; Novo Nordisk A/S (Novo Nordisk, Bagsvaerd, Denmark) [NNC 55-0118, NN414]en_US
dc.description.sponsorshipThis work was supported by Grants from TUBI-TAK, 109S516 and 110S397 (Turkey). We thank Novo Nordisk A/S (Novo Nordisk, Bagsvaerd, Denmark) for providing NNC 55-0118 and NN414.en_US
dc.identifier.citationBal, R., Öztürk, G., Önalan Etem, E., Him, A., Cengiz, N., Kulo?lu, T. ... Tektemur, A. (2018). Modulation of excitability of stellate neurons in the ventral cochlear nucleus of mice by atp-sensitive potassium channels. Journal of Membrane Biology, 251(1), 163-178. https://dx.doi.org/10.1007/s00232-017-0011-x
dc.identifier.doi10.1007/s00232-017-0011-x
dc.identifier.endpage178
dc.identifier.issn0022-2631
dc.identifier.issn1432-1424
dc.identifier.issue1
dc.identifier.scopusqualityQ2
dc.identifier.startpage163
dc.identifier.urihttps://dx.doi.org/10.1007/s00232-017-0011-x
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2100
dc.identifier.volume251
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ecinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/109S516 110S397
dc.relation.ispartofJournal of Membrane Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectStellate Cells
dc.subjectCochlear Nucleus
dc.subjectATP-Sensitive Potassium Channels
dc.subjectK-ATP
dc.titleModulation of excitability of stellate neurons in the ventral cochlear nucleus of mice by atp-sensitive potassium channels
dc.typeArticle

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