The role of TNF-? and its target HSP-70 in triggering apoptosis in normozoospermic and non-normozoospermic samples

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dc.authorid0000-0003-3302-5004
dc.authorid0000-0001-6698-9032
dc.authorid0000-0002-7059-1884
dc.contributor.authorKarabulut, Seda
dc.contributor.authorGürsoy Gürgen, Duygu
dc.contributor.authorKutlu, Pelin
dc.contributor.authorKeskin, İlknur
dc.date.accessioned2023-01-09T07:52:09Z
dc.date.available2023-01-09T07:52:09Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.description.abstractObjective: Semen analysis is performed as one of the screening tests for infertility, including motility, morphology, and concentration observation. We aimed to investigate the expression rates of tumor necrosis factor-? (TNF-?) and heat shock protein (HSP)-70 as two opposite affectors of apoptosis in men with normal semen parameters and abnormal parameters to find the possible effect of this pathway on sperm parameters. We also aimed to investigate the apoptotic markers (DNA fragmentation and Caspase-3 expression) to observe the correlation of this pathway with apoptosis. Materials and Methods: A total of 32 men who applied for infertility evaluation were included in the study. Semen analysis was performed according to WHO criteria. Liquefaction time, appearance, volume, pH, viscosity, sperm concentration, total motility rate, sperm motility, and percentage of spermatozoa with normal morphology were determined. TNF-?, HSP-70, and Caspase-3 immunolocalization were scored histologically. A sperm chromatin dispersion test was used to observe DNA fragmentation. Results: There was no significant difference in TNF-? protein expression rate (mild level). The HSP-70 expression rate was lower, especially in the head region of normo. Caspase-3 was higher totally in non-normo. DNA fragmentation levels were similar in both the groups. Conclusion: From TNF-? protein expression at the mild level in both the groups, it may be hypothesized that the apoptotic pathway might not be triggered by the extrinsic pathway. We found a negative correlation between HSP-70 and Caspase-3 expressions, providing further evidence that HSP-70 works as an inhibitor to apoptosis. This, particularly on specific points, made us think the communication might begin in the anterior chamber, then flow through the cell body to the tail. HSP-70 expression was lower in normo than in non-normo, indicating the possible role of HSP-70 as an answer to any type of stressor in non-normozoospermic patients. Correspondingly, it may be concluded that HSP has an antiapoptotic effect, causing inhibition in the elimination of abnormal sperm cells impairing sperm parameters.
dc.identifier.citationKarabulut, S., Gürsoy Gürgen, D., Kutlu, P. ve Keskin, İ. (2022). The role of TNF-? and its target HSP-70 in triggering apoptosis in normozoospermic and non-normozoospermic samples. Biopreservation and Biobanking, 20(6), 485-492. https://dx.doi.org/10.1089/bio.2021.0056
dc.identifier.doi10.1089/bio.2021.0056
dc.identifier.endpage492
dc.identifier.issn1947-5535
dc.identifier.issn1947-5543
dc.identifier.issue6
dc.identifier.pmid35652686
dc.identifier.scopus2-s2.0-85144589397
dc.identifier.scopusqualityQ2
dc.identifier.startpage485
dc.identifier.urihttps://dx.doi.org/10.1089/bio.2021.0056
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10262
dc.identifier.volume20
dc.identifier.wos000806604100001en_US
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKarabulut, Seda
dc.institutionauthorGürsoy Gürgen, Duygu
dc.institutionauthorKeskin, İlknur
dc.language.isoen
dc.publisherNLM (Medline)
dc.relation.ispartofBiopreservation and Biobankingen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApoptosis
dc.subjectCaspase-3
dc.subjectHSP-70
dc.subjectImmunolocation
dc.subjectInfertility
dc.subjectTNF-?
dc.titleThe role of TNF-? and its target HSP-70 in triggering apoptosis in normozoospermic and non-normozoospermic samples
dc.title.alternativeThe role of TNF-alpha and its target HSP-70 in triggering apoptosis in normozoospermic and non-normozoospermic samples
dc.typeArticle

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