Chronic unpredictable stress disturbs the blood-testis barrier affecting sperm parameters in mice

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dc.authorid0000-0001-7933-4262
dc.authorid0000-0003-1526-2065
dc.authorid0000-0003-3302-5004
dc.authorid0000-0001-7446-472X
dc.authorid0000-0002-8662-5284
dc.authorid0000-0003-2350-6381
dc.authorid0000-0002-7059-1884
dc.contributor.authorKolbaşı, Bircan
dc.contributor.authorBülbül, Muhammet Volkan
dc.contributor.authorKarabulut, Seda
dc.contributor.authorErdem Altun, Ceren
dc.contributor.authorÇakıcı, Çağrı
dc.contributor.authorÜlfer, Gözde
dc.contributor.authorMüdok, Tangül
dc.contributor.authorKeskin, İlknur
dc.date.accessioned2021-05-27T06:49:05Z
dc.date.available2021-05-27T06:49:05Z
dc.date.issued2021
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractResearch question: Does chronic stress affect the key proteins and sperm parameters of the blood & ndash;testis barrier (BTB)? Design: C57Bl/6 mice were divided into two groups: a non-treated control group and a chronic unpredictable stress (CUS) applied group. The stress status of the animals was confirmed with behavioural tests. Histopathologic evaluation was conducted by haematoxylin and eosin staining and electron microscope. Malondialdehyde, corticosterone and testosterone levels were evaluated in peripheral blood. Expression levels of BTB proteins, namely zonula occludens-1 (ZO-1), claudin-11 (CLDN11) and clathrin in Sertoli cells, were assessed by Western blotting and immunofluorescence techniques. Sperm samples were collected from cauda epididymis, and sperm parameters analysed. Results: The stress model was confirmed by behavioural tests. Histopathological evaluation of the testes demonstrated a mild degeneration in seminiferous tubules. Malondialdehyde (P = 0.008) and corticosterone levels increased (P = 0.004) and testosterone levels decreased (P = 0.005) in the CUS group. Electron microscopic evaluation confirmed the damage in BTB integrity in the CUS group. Western blot analysis showed that ZO-1 and CLDN11 levels were significantly decreased, although clathrin levels were unchanged. Although sperm concentration and total motility rate were not significantly different between the groups, progressive motility (P = 0.03), normal sperm morphology (P = 0.04), chromatin integrity (toluidine blue) (P = 0.002) and the acrosomal reaction rate (P = 0.002) were significantly decreased, and acrosomal abnormality rate was dramatically increased (P = 0.04) in the CUS group. Conclusions: In mice, CUS disrupted BTB integrity and impaired sperm parameters. A decrease in ZO-1 and CLDN11 expression levels may be proposed as the causative factor.
dc.identifier.citationKolbaşı, B., Bülbül, M. V., Karabulut, S., Erdem Altun, C., Çakıcı, Ç., Ülfer, G. ... Keskin, İ. (2021). Chronic unpredictable stress disturbs the blood-testis barrier affecting sperm parameters in mice. Reproductive BioMedicine Online, 42(5), 983-995. https://dx.doi.org/10.1016/j.rbmo.2020.12.007
dc.identifier.doi10.1016/j.rbmo.2020.12.007
dc.identifier.endpage995
dc.identifier.issn1472-6483
dc.identifier.issn1472-6491
dc.identifier.issue5
dc.identifier.scopusqualityQ1
dc.identifier.startpage983
dc.identifier.urihttps://dx.doi.org/10.1016/j.rbmo.2020.12.007
dc.identifier.urihttps://hdl.handle.net/20.500.12511/6942
dc.identifier.volume42
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Ltd
dc.relation.ispartofReproductive BioMedicine Onlineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectBlood-Testis Barrier
dc.subjectChronic Unpredictable Stress
dc.subjectSperm
dc.subjectSpermatogenesis
dc.subjectTestis
dc.titleChronic unpredictable stress disturbs the blood-testis barrier affecting sperm parameters in mice
dc.typeArticle

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