Comparison of protein expression profile of limb regeneration between neotenic and metamorphic axolotl

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dc.authorid0000-0002-6183-9489
dc.authorid0000-0003-0352-1947
dc.contributor.authorSibai, Mustafa
dc.contributor.authorAltuntaş, Ebru
dc.contributor.authorSüzek, Barış Ethem
dc.contributor.authorŞahin, Betül
dc.contributor.authorParlayan, Cüneyd
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorBaykal, Ahmet Tarık
dc.contributor.authorDemircan, Turan
dc.date.accessioned2019-12-27T06:12:43Z
dc.date.available2019-12-27T06:12:43Z
dc.date.issued2020
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.description.abstractThe axolotl (Ambystoma mexicanum) salamander, a urodele amphibian, has an exceptional regenerative capacity to fully restore an amputated limb throughout the life-long lasting neoteny. By contrast, when axolotls are experimentally induced to metamorphosis, attenuation of the limb's regenerative competence is noticeable. Here, we sought to discern the proteomic profiles of the early stages of blastema formation of neotenic and metamorphic axolotls after limb amputation by employing LC-MS/MS technology. We quantified a total of 714 proteins and qRT-PCR for selected genes was performed to validate the proteomics results and provide evidence for the putative link between immune system activity and regenerative potential. This study provides new insights for examination of common and distinct molecular mechanisms in regeneration-permissive neotenic and regeneration-deficient metamorphic stages at the proteome level.
dc.identifier.citationSibai, M., Altuntaş, E., Süzek, B. E., Şahin, B., Parlayan, C., Öztürk, G. ... Demircan, T. (2020). Comparison of protein expression profile of limb regeneration between neotenic and metamorphic axolotl. Biochemical and Biophysical Research Communications, 522(2), 428-434. http://doi.org/10.1016/j.bbrc.2019.11.118
dc.identifier.doi10.1016/j.bbrc.2019.11.118
dc.identifier.endpage434
dc.identifier.issn0006-291X
dc.identifier.issn1090-2104
dc.identifier.issue2
dc.identifier.scopusqualityQ1
dc.identifier.startpage428
dc.identifier.urihttp://doi.org/10.1016/j.bbrc.2019.11.118
dc.identifier.urihttps://hdl.handle.net/20.500.12511/4749
dc.identifier.volume522
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectProteomics
dc.subjectAxolotl
dc.subjectLimb Regeneration
dc.subjectMetamorphosis
dc.subjectGene Expression Profile
dc.titleComparison of protein expression profile of limb regeneration between neotenic and metamorphic axolotl
dc.typeArticle

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