Methodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: Sequential sampling, length of sampling time and two different sampling methods

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dc.contributor.authorKayar, Nezehat Arzu
dc.contributor.authorOduncuoğlu, Bahar Füsun
dc.contributor.authorHaliloğlu, Seyfullah
dc.contributor.authorSerpek, Behiç
dc.contributor.authorAtaoğlu, Tamer
dc.contributor.authorAlptekin, Nilgün Özlem
dc.date.accessioned2020-01-03T06:54:38Z
dc.date.available2020-01-03T06:54:38Z
dc.date.issued2020
dc.departmentİstanbul Medipol Üniversitesi, Diş Hekimliği Fakültesi, Periodontoloji Ana Bilim Dalı
dc.description.abstractObjectives: The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme levels by using intracrevicular and orifice methods. Material and methods: Twenty adults (mean age of 41.8 years, ranged 31-60 years, 18 males and 2 females) with chronic periodontitis were enrolled and all completed the 3-d study. GCF was collected by both intracrevicular and intrasulcular methods, 720 samples of GCF were collected. In first, second and third day, the length of sampling time in seconds (s) and order were '5- 10-30-s'; '10- 30- 5-s' and '30- 5- 10-s,' respectively. GCF elastase levels were determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide. Results: NE activity (mu U) and NE activity/volume (mu U/mu l) were significantly different for order of sampling (p < .05), but not for the length of sampling time (p>.05). Conclusions: Within the limits of this study, the choice of sampling technique in GCF-profile studies seems to be a critical decision as it has the potential to affect the GCF volume and NE activity.
dc.identifier.citationKayar, N. A., Oduncuoğlu, B. F., Haliloğlu, S., Serpek, B., Ataoğlu, T. ve Alptekin, N. Ö. (2020). Methodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: Sequential sampling, length of sampling time and two different sampling methods. Acta Odontologica Scandinavica, 78(4), 290-296. https://doi.org/10.1080/00016357.2019.1703141
dc.identifier.doi10.1080/00016357.2019.1703141
dc.identifier.endpage296
dc.identifier.issn0001-6357
dc.identifier.issn1502-3850
dc.identifier.issue4
dc.identifier.scopusqualityQ1
dc.identifier.startpage290
dc.identifier.urihttps://doi.org/10.1080/00016357.2019.1703141
dc.identifier.urihttps://hdl.handle.net/20.500.12511/4883
dc.identifier.volume78
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor and Francis Ltd
dc.relation.ispartofActa Odontologica Scandinavicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectGingival Crevicular Fluid
dc.subjectSampling Methods
dc.subjectSequential Sampling
dc.subjectSampling
dc.titleMethodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: Sequential sampling, length of sampling time and two different sampling methods
dc.typeArticle

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