Effects of bone surface topography and chemistry on macrophage polarization

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dc.authorid0000-0002-3178-3955
dc.contributor.authorÖzçolak, Birgün
dc.contributor.authorErenay, Berkay
dc.contributor.authorOdabaş, Sedat
dc.contributor.authorJandt, Klaus D.
dc.contributor.authorGaripcan, Bora
dc.date.accessioned2024-06-12T10:59:04Z
dc.date.available2024-06-12T10:59:04Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.description.abstractSurface structure plays a crucial role in determining cell behavior on biomaterials, influencing cell adhesion, proliferation, differentiation, as well as immune cells and macrophage polarization. While grooves and ridges stimulate M2 polarization and pits and bumps promote M1 polarization, these structures do not accurately mimic the real bone surface. Consequently, the impact of mimicking bone surface topography on macrophage polarization remains unknown. Understanding the synergistic sequential roles of M1 and M2 macrophages in osteoimmunomodulation is crucial for effective bone tissue engineering. Thus, exploring the impact of bone surface microstructure mimicking biomaterials on macrophage polarization is critical. In this study, we aimed to sequentially activate M1 and M2 macrophages using Poly-L-Lactic acid (PLA) membranes with bone surface topographical features mimicked through the soft lithography technique. To mimic the bone surface topography, a bovine femur was used as a model surface, and the membranes were further modified with collagen type-I and hydroxyapatite to mimic the bone surface microenvironment. To determine the effect of these biomaterials on macrophage polarization, we conducted experimental analysis that contained estimating cytokine release profiles and characterizing cell morphology. Our results demonstrated the potential of the hydroxyapatite-deposited bone surface-mimicked PLA membranes to trigger sequential and synergistic M1 and M2 macrophage polarizations, suggesting their ability to achieve osteoimmunomodulatory macrophage polarization for bone tissue engineering applications. Although further experimental studies are required to completely investigate the osteoimmunomodulatory effects of these biomaterials, our results provide valuable insights into the potential advantages of biomaterials that mimic the complex microenvironment of bone surfaces.
dc.identifier.citationÖzçolak, B., Erenay, B., Odabaş, S., Jandt, K. D. ve Garipcan, B. (2024). Effects of bone surface topography and chemistry on macrophage polarization. Scientific Reports, 14(1), 12721. http://dx.doi.org/10.1038/s41598-024-62484-3
dc.identifier.doi10.1038/s41598-024-62484-3
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.pmid38830871
dc.identifier.scopus2-s2.0-85195007479
dc.identifier.scopusqualityQ1
dc.identifier.startpage12721
dc.identifier.urihttp://dx.doi.org/10.1038/s41598-024-62484-3
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12632
dc.identifier.volume14
dc.identifier.wos001238391600027en_US
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzçolak, Birgün
dc.language.isoen
dc.relation.ispartofScientific Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiomimetic
dc.subjectBone Surface Topography
dc.subjectMacrophage Polarization
dc.subjectMacrophages
dc.subjectSoft Lithography
dc.subjectSurface Modification Osteoimmunomodulation
dc.titleEffects of bone surface topography and chemistry on macrophage polarization
dc.typeArticle

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